PUBLICATION

The role of CRH in behavioral responses to acute restraint stress in zebrafish

Authors
Ghisleni, G., Capiotti, K.M., Da Silva, R.S., Oses, J.P., Piato, A.L., Soares, V., Bogo, M.R., and Bonan, C.D.
ID
ZDB-PUB-110907-40
Date
2012
Source
Progress in neuro-psychopharmacology & biological psychiatry   36(1): 176-82 (Journal)
Registered Authors
Bonan, Carla Denise
Keywords
acute restraint stress, corticotropin-releasing hormone, cortisol, locomotor activity, zebrafish
MeSH Terms
  • Animals
  • RNA, Messenger/biosynthesis
  • Zebrafish
  • Acute Disease
  • Hydrocortisone/biosynthesis
  • Stress, Psychological/genetics
  • Stress, Psychological/metabolism*
  • Stress, Psychological/psychology*
  • Restraint, Physical/adverse effects
  • Corticotropin-Releasing Hormone/genetics
  • Corticotropin-Releasing Hormone/physiology*
  • Gene Expression Regulation
(all 12)
PubMed
21893154 Full text @ Prog. Neuropsychopharmacol. Biol. Psychiatry
Abstract
In teleosts, changes in swimming, exploring, general locomotor activity, and anxious state can be a response to stress mediated by the corticotropin-releasing hormone system activation and its effects on glucocorticoid levels. Zebrafish has been widely used to study neuropharmacology and has become a promising animal model to investigate neurobehavioral mechanisms of stress. In this report the animals were submitted to acute restraint stress for different time lengths (15, 60 and 90 min) for further evaluation of behavioral patterns, whole-body cortisol content, and corticotropin-releasing hormone expression. The results demonstrated an increase in the locomotor activity and an alteration in the swimming pattern during a 5-min trial after the acute restraint stress. Interestingly, all groups of fish tested in the novel tank test exhibited signs of anxiety as evaluated by the time spent in the bottom of the tank. Whole-body cortisol content showed a positive correlation with increased behavioral indices of locomotion in zebrafish whereas molecular analysis of corticotropin-releasing hormone showed a late reduction of mRNA expression (90 min). Altogether, we present a model of acute restraint stress in zebrafish, confirmed by elevated cortisol content, as a valid and reliable model to study the biochemical basis of stress behavior, which seems to be accompanied by a negative feed-back of corticotropin-release hormone mRNA expression.
Genes / Markers
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
No data available
Orthology
Engineered Foreign Genes
No data available
Mapping
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Citation
Ghisleni, G., Capiotti, K.M., Da Silva, R.S., Oses, J.P., Piato, A.L., Soares, V., Bogo, M.R., and Bonan, C.D. (2012) The role of CRH in behavioral responses to acute restraint stress in zebrafish. Progress in neuro-psychopharmacology & biological psychiatry. 36(1):176-82.