PUBLICATION

Expression and regulation by thyroid hormone (TH) of zebrafish IGF-I gene and amphioxus IGFl gene with implication of the origin of TH/IGF signaling pathway

Authors
Wang, Y., and Zhang, S.
ID
ZDB-PUB-110901-14
Date
2011
Source
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology   160(4): 474-9 (Journal)
Registered Authors
Keywords
amphioxus, zebrafish, liver, thyroid hormone, IGF
MeSH Terms
  • Animals
  • Chordata/genetics
  • Chordata/metabolism*
  • Chordata/physiology
  • Gene Expression Profiling
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish/physiology
  • Insulin-Like Growth Factor I/genetics
  • Insulin-Like Growth Factor I/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Somatomedins/genetics
  • Somatomedins/metabolism*
  • Gene Expression Regulation
  • Liver/metabolism
  • Signal Transduction
  • Thyroid Hormones/metabolism*
  • Thyroid Hormones/pharmacology
(all 19)
PubMed
21867768 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Abstract
Thyroid hormone (TH)/insulin-like growth factor (IGF) signaling pathway has been identified in all the vertebrates, but its evolutionary origin remains elusive. In this study we examined the expression profiles in vitro as well as in vivo of the IGF-I gene of fish Danio rerio (vertebrate) and the IGF-like gene (IGFl) of amphioxus Branchiostoma japonicum (protochordate) following T3 treatment. Our results showed that T3 was able to enhance hepatic IGF-I/IGFl gene expression in vitro in both zebrafish and amphioxus in a dose-dependent manner. This T3-induced hepatic expression of IGF-I/IGFl genes in both species was significantly inhibited by the T3-specific inhibitor DEA, indicating the specificity of IGF-I/IGFl gene regulation by T3. At 100 nM T3, in both the long (42 h) and short (8 h) time course experiments, the IGF-I/IGFl gene expression profiles following T3 treatment in the tissue cultures of both species exhibited closely similar pattern and trend. Moreover, exposure of zebrafish and amphioxus to T3 in vivo for 72 h induced a significant increase in the expression of IGF-I/IGFl genes in both the liver and the hepatic caecum. These data together suggest that amphioxus and zebrafish both share a similar regulatory mechanism of IGF gene expression in response to T3, providing an evidence for the presence of a vertebrate-like TH/IGF signaling pathway in the protochordate amphioxus.
Genes / Markers
Figures
No images available
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Wang, Y., and Zhang, S. (2011) Expression and regulation by thyroid hormone (TH) of zebrafish IGF-I gene and amphioxus IGFl gene with implication of the origin of TH/IGF signaling pathway. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 160(4):474-9.