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ZFIN ID: ZDB-PUB-110811-37
Differential calcium signaling by cone specific guanylate cyclase-activating proteins from the zebrafish retina
Scholten, A., and Koch, K.W.
Date: 2011
Source: PLoS One   6(8): e23117 (Journal)
Registered Authors:
Keywords: none
MeSH Terms:
  • Animals
  • Base Sequence
  • Calcium/metabolism
  • Calcium Signaling*
  • Chromatography, High Pressure Liquid
  • Cloning, Molecular
  • DNA Primers
  • Enzyme Activation
  • Guanylate Cyclase/metabolism
  • Guanylate Cyclase-Activating Proteins/genetics
  • Guanylate Cyclase-Activating Proteins/metabolism
  • Guanylate Cyclase-Activating Proteins/physiology*
  • Hydrolysis
  • Mutagenesis, Site-Directed
  • Polymerase Chain Reaction
  • Protein Binding
  • Protein Conformation
  • Retina/metabolism*
  • Spectrometry, Fluorescence
  • Zebrafish
PubMed: 21829700 Full text @ PLoS One
Zebrafish express in their retina a higher number of guanylate cyclase-activating proteins (zGCAPs) than mammalians pointing to more complex guanylate cyclase signaling systems. All six zGCAP isoforms show distinct and partial overlapping expression profiles in rods and cones. We determined critical Ca2+-dependent parameters of their functional properties using purified zGCAPs after heterologous expression in E.coli. Isoforms 1–4 were strong, 5 and 7 were weak activators of membrane bound guanylate cyclase. They further displayed different Ca2+-sensitivities of guanylate cyclase activation, which is half maximal either at a free Ca2+ around 30 nM (zGCAP1, 2 and 3) or around 400 nM (zGCAP4, 5 and 7). Zebrafish GCAP isoforms showed also differences in their Ca2+/Mg2+-dependent conformational changes and in the Ca2+-dependent monomer-dimer equilibrium. Direct Ca2+-binding revealed that all zGCAPs bound at least three Ca2+. The corresponding apparent affinity constants reflect binding of Ca2+ with high (d100 μM), medium (0.1–5 μM) and/or low (e5 μM) affinity, but were unique for each zGCAP isoform. Our data indicate a Ca2+-sensor system in zebrafish rod and cone cells supporting a Ca2+-relay model of differential zGCAP operation in these cells.