PUBLICATION
A zebrafish scale assay to monitor dioxin-like activity in surface water samples
- Authors
- Pelayo, S., López-Roldan, R., González, S., Casado, M., Raldua, D., Cortina, J.L., and Pina, B.
- ID
- ZDB-PUB-110811-25
- Date
- 2011
- Source
- Analytical and bioanalytical chemistry 401(6): 1861-9 (Journal)
- Registered Authors
- Keywords
- dioxine-like pollutants, real-time PCR, danio rerio, bioassays, RNA quantification, cyp1a, GC-MS
- MeSH Terms
- none
- PubMed
- 21822775 Full text @ Anal. Bioanal. Chem.
Citation
Pelayo, S., López-Roldan, R., González, S., Casado, M., Raldua, D., Cortina, J.L., and Pina, B. (2011) A zebrafish scale assay to monitor dioxin-like activity in surface water samples. Analytical and bioanalytical chemistry. 401(6):1861-9.
Abstract
New regulations on water quality require a close control of the possible biological activities known or unexpected pollutants
may bring about. We present here a protocol based on the direct exposure of zebrafish to river water and the analysis of expression
of specific genes in their scales to determine the presence of compounds with dioxin-like biological activity. The method
does not require the killing of animals and allows detection of the biological activity after a single day of exposure. When
tested, the method with real samples from the Llobregat River, clear temporal and spatial variations were observed, demonstrating
its suitability for monitoring natural variations in water quality linked to specific discharges. High biological activities
were unrelated to the currently checked water quality parameters (macropollutants, turbidity, TOC, etc.), but they did correlate
with the presence of micropollutants (estrogens, detergents, etc.) related to domestic and/or industrial runoffs. The scale
assay therefore provides a new tool to evaluate water quality changes that cannot be easily derived from the existing standard
analytical procedures. It ranks among the very few described protocols able to detect biological effects from natural water
samples, without a pre-concentration step, and after only 24 h of exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping