PUBLICATION
Molecular characterization of growth differentiation factor 9 and its spatio-temporal expression pattern in gibel carp (Carassius auratus gibelio)
- Authors
- Liu, Z., Chen, A., Yang, Z., Wei, H., and Leng, X.
- ID
- ZDB-PUB-110803-2
- Date
- 2012
- Source
- Molecular biology reports 39(4): 3863-3870 (Journal)
- Registered Authors
- Keywords
- carassius auratus gibelio, GDF9, gene expression, human chorionic gonadotrophin, immunohistochemistry, ovary
- MeSH Terms
-
- Animals
- Carps/genetics*
- Chorionic Gonadotropin/pharmacology
- Cloning, Molecular
- DNA, Complementary/genetics
- Female
- Gene Expression Regulation*/drug effects
- Growth Differentiation Factor 9/genetics*
- Growth Differentiation Factor 9/metabolism
- Humans
- Immunohistochemistry
- Ovary/cytology
- Ovary/drug effects
- Ovary/metabolism
- Protein Transport
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction
- Time Factors
- PubMed
- 21779806 Full text @ Mol. Biol. Rep.
Citation
Liu, Z., Chen, A., Yang, Z., Wei, H., and Leng, X. (2012) Molecular characterization of growth differentiation factor 9 and its spatio-temporal expression pattern in gibel carp (Carassius auratus gibelio). Molecular biology reports. 39(4):3863-3870.
Abstract
Growth differentiation factor 9 (GDF9) is a member of the transforming growth factor β (TGF-β) superfamily with a key role in regulating follicle development. In this study, the GDF9 full-length genomic DNA and cDNA were isolated and characterized from the gibel carp ovary using rapid-amplification of cDNA ends (RACE) and LD-PCR. The full-length genomic DNA and cDNA sequences of GDF9 are 3979 and 2044 bp which code 428 amino acid residues with a specific RKKR protease cleavage site of TGF-β superfamily. Sequence analysis showed that gibel carp was similar to zebrafish and other fish species. Spatio-temporal expression analysis using real-time quantitative PCR revealed that GDF9 mRNA was largely expressed in ovary and testis. GDF9 is mainly present at stage I follicles indicating its important role in early follicles development. The same result was obtained in immunohistochemistry localization of GDF9 protein. Within the follicle, the follicle layer cells were barely expressed whereas GDF9 mRNA was mostly expressed in the oocytes. Supplemented with human chorionic gonadotropin (hCG) in isolated follicles, the expression of GDF9 mRNA was increased firstly and then decreased. The results of this study indicated that GDF9 gene played a role in fish during development of follicles, especially in the early stage follicles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping