PUBLICATION
Injecting zebrafish with DNA or RNA constructs encoding fluorescent protein reporters
- Authors
- Godinho, L.
- ID
- ZDB-PUB-110713-8
- Date
- 2011
- Source
- Cold Spring Harbor protocols 2011(7): 871-4 (Journal)
- Registered Authors
- Godinho, Leanne
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA/genetics*
- Developmental Biology/methods*
- Luminescent Proteins/biosynthesis*
- Luminescent Proteins/genetics
- Morphogenesis
- RNA/genetics*
- Staining and Labeling/methods*
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 21724811 Full text @ Cold Spring Harb. Protoc.
Citation
Godinho, L. (2011) Injecting zebrafish with DNA or RNA constructs encoding fluorescent protein reporters. Cold Spring Harbor protocols. 2011(7):871-4.
Abstract
In the last two decades, the small tropical freshwater-dwelling zebrafish has emerged as a popular model for developmental biologists. The exceptional optical accessibility of zebrafish embryos coupled with the ability to use genetic tools to indelibly tag cells have been exploited to watch developmental events as they unfold. Moreover, because zebrafish are vertebrates, the knowledge gained through such studies is broadly applicable. Injecting zebrafish with constructs that encode fluorescent proteins is a versatile method for labeling cells and tissues for imaging. The method, described here, can be used to generate transiently or stably transfected organisms showing ubiquitous or mosaic patterns of fluorescence.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping