PUBLICATION

Constructing a microinjection chamber for zebrafish eggs

Authors
Godinho, L.
ID
ZDB-PUB-110713-7
Date
2011
Source
Cold Spring Harbor protocols   2011(7): 875-6 (Journal)
Registered Authors
Godinho, Leanne
Keywords
none
MeSH Terms
  • Animals
  • Developmental Biology/methods*
  • Microinjections/methods*
  • Ovum*
  • Staining and Labeling/methods*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed
21724810 Full text @ Cold Spring Harb. Protoc.
Abstract
In the last two decades, the small tropical freshwater-dwelling zebrafish has emerged as a popular model for developmental biologists. The exceptional optical accessibility of zebrafish embryos coupled with the ability to use genetic tools to indelibly tag cells have been exploited to watch developmental events as they unfold. Moreover, because zebrafish are vertebrates, the knowledge gained through such studies is broadly applicable. This protocol describes the construction of a microinjection chamber for zebrafish eggs. Several microinjection chambers can be made, can be stored at 4°C, and can be used for several weeks until the injection trenches become damaged through repeated use and/or mold begins to grow on the agarose.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes