PUBLICATION
Constructing a microinjection chamber for zebrafish eggs
- Authors
- Godinho, L.
- ID
- ZDB-PUB-110713-7
- Date
- 2011
- Source
- Cold Spring Harbor protocols 2011(7): 875-6 (Journal)
- Registered Authors
- Godinho, Leanne
- Keywords
- none
- MeSH Terms
-
- Animals
- Developmental Biology/methods*
- Microinjections/methods*
- Ovum*
- Staining and Labeling/methods*
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 21724810 Full text @ Cold Spring Harb. Protoc.
Citation
Godinho, L. (2011) Constructing a microinjection chamber for zebrafish eggs. Cold Spring Harbor protocols. 2011(7):875-6.
Abstract
In the last two decades, the small tropical freshwater-dwelling zebrafish has emerged as a popular model for developmental biologists. The exceptional optical accessibility of zebrafish embryos coupled with the ability to use genetic tools to indelibly tag cells have been exploited to watch developmental events as they unfold. Moreover, because zebrafish are vertebrates, the knowledge gained through such studies is broadly applicable. This protocol describes the construction of a microinjection chamber for zebrafish eggs. Several microinjection chambers can be made, can be stored at 4°C, and can be used for several weeks until the injection trenches become damaged through repeated use and/or mold begins to grow on the agarose.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping