PUBLICATION
Ethanolic Extract of Fructus Alpinia oxyphylla Protects Against 6-Hydroxydopamine-Induced Damage of PC12 Cells In Vitro and Dopaminergic Neurons in Zebrafish
- Authors
- Zhang, Z.J., Cheang, L.C., Wang, M.W., Li, G.H., Chu, I.K., Lin, Z.X., and Lee, S.M.
- ID
- ZDB-PUB-110713-62
- Date
- 2012
- Source
- Cellular and molecular neurobiology 32(1): 27-40 (Journal)
- Registered Authors
- Keywords
- fructus Alpina oxyphylla, neuroprotection, Parkinson's disease, zebrafish, PC12 cell
- MeSH Terms
-
- Cytoprotection/drug effects*
- Larva/drug effects
- Larva/growth & development
- Larva/physiology
- PC12 Cells
- Zebrafish/embryology
- Zebrafish/growth & development
- Embryo, Nonmammalian
- Animals
- Locomotion/drug effects
- Ethanol/pharmacology
- Dopaminergic Neurons/drug effects*
- Dopaminergic Neurons/physiology
- Plant Extracts/chemistry
- Plant Extracts/pharmacology*
- Rats
- Behavior, Animal/drug effects
- Cell Death/drug effects
- Oxidopamine/toxicity*
- PubMed
- 21744117 Full text @ Cell. Mol. Neurobiol.
Citation
Zhang, Z.J., Cheang, L.C., Wang, M.W., Li, G.H., Chu, I.K., Lin, Z.X., and Lee, S.M. (2012) Ethanolic Extract of Fructus Alpinia oxyphylla Protects Against 6-Hydroxydopamine-Induced Damage of PC12 Cells In Vitro and Dopaminergic Neurons in Zebrafish. Cellular and molecular neurobiology. 32(1):27-40.
Abstract
In an attempt to understand the neuroprotective effect of Fructus Alpinia oxyphylla (AOE) and to elucidate its underlying mechanism of action, the ethanolic extract of AOE was investigated using zebrafish
and PC12 cell models. AOE prevented and restored 6-hydroxydopamine (6-OHDA)-induced dopaminergic (DA) neuron degeneration
and attenuated a deficit of locomotor activity in a zebrafish (Danio rerio) model of Parkinson’s disease (PD). Treatment with AOE increased the viability of 6-OHDA-treated PC12 cells in vitro in a
dose-dependent manner by attenuating cellular apoptosis. However, protocatechuic acid (PCA) and chrysin, two known polyphenol
components of AOE, could not reproduce the neuroprotective activity of AOE in the PD zebrafish or PC12 cell models. A mechanistic
study found that the protective effect of AOE against 6-OHDA-induced neuronal injury involved anti-inflammatory action (down-regulation
of gene expression of IL-1β and TNF-α) and anti-oxidative action (inhibition of NO production and iNOS expression in PC12
cells). Moreover, the PI3K-AKT pathway might be part of the mechanism of neuroprotection of AOE. The results of this research
are expected to provide a scientific rationale for the use of AOE in the treatment of PD. However, it is important that the
active components that contribute to the neuroprotective action of AOE are identified and characterized.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping