PUBLICATION

Using intravital dyes to ubiquitously label embryonic zebrafish

Authors
Godinho, L.
ID
ZDB-PUB-110713-6
Date
2011
Source
Cold Spring Harbor protocols   2011(7): 877-8 (Journal)
Registered Authors
Godinho, Leanne
Keywords
none
MeSH Terms
  • Animals
  • Developmental Biology/methods*
  • Fluorescent Dyes/metabolism*
  • Luminescent Proteins/biosynthesis*
  • Morphogenesis
  • Staining and Labeling/methods*
  • Zebrafish/embryology*
PubMed
21724809 Full text @ Cold Spring Harb. Protoc.
Abstract
In the last two decades, the small tropical freshwater-dwelling zebrafish has emerged as a popular model for developmental biologists. The exceptional optical accessibility of zebrafish embryos coupled with the ability to use genetic tools to indelibly tag cells have been exploited to watch developmental events as they unfold. Moreover, because zebrafish are vertebrates, the knowledge gained through such studies is broadly applicable. This protocol describes the use of intravital dyes to ubiquitously label embryonic zebrafish. Intravital dyes such as CellTrace BODIPY TR methyl ester can be used to counterstain embryos that express fluorescent proteins either through transient injections or by stable transgenics. BODIPY TR should only be used in combination with fluorescent proteins that can be spectrally separated from it. The BODIPY TR absorption spectrum peaks at 598 nm, and its emission spectrum peaks at 625 nm. Embryos should be stained just before imaging.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes