PUBLICATION
Transforming growth factor-beta (TGF-β) & keratocyte motility in 24-hour zebrafish explant cultures
- Authors
- Tan, B., Pascual, A., de Beus, A., Cooper, K., and Hull, E.
- ID
- ZDB-PUB-110713-22
- Date
- 2011
- Source
- Cell biology international 35(11): 1131-9 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Migration Assays
- Cell Movement
- Cells, Cultured
- Culture Media, Serum-Free/chemistry
- Enzyme-Linked Immunosorbent Assay
- Fibroblasts/metabolism*
- Fibroblasts/physiology
- Gene Expression Regulation
- Humans
- Protein Serine-Threonine Kinases/genetics
- Protein Serine-Threonine Kinases/metabolism*
- RNA, Messenger/analysis
- RNA, Messenger/metabolism
- Receptors, Transforming Growth Factor beta/genetics
- Receptors, Transforming Growth Factor beta/metabolism*
- Serum/metabolism
- Time Factors
- Transforming Growth Factor beta1/metabolism*
- Transforming Growth Factor beta1/pharmacology
- Up-Regulation
- Zebrafish/metabolism
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 21729005 Full text @ Cell Biol. Int.
Citation
Tan, B., Pascual, A., de Beus, A., Cooper, K., and Hull, E. (2011) Transforming growth factor-beta (TGF-β) & keratocyte motility in 24-hour zebrafish explant cultures. Cell biology international. 35(11):1131-9.
Abstract
Fish keratocytes are used as a model system for the study of the mechanics of cell motility because of their characteristic rapid, smooth gliding motion but little work has been done on the regulation of fish keratocyte movement. As TGF-β plays multiple roles in primary human keratinocyte cell migration, we investigated the possible involvement of TGF-β in fish keratocyte migration. Studying the involvement of TGF-β1 in 24-hour keratocyte explant allows examination of the cells before alterations in cellular physiology occur due to extended culture times. During this initial period, TGF-β levels increase 6.2 fold in serum free media (SFM) and 2.4 fold in SFM + 2% fetal bovine serum (FBS) while TGF-β1 and TGFβRII mRNA levels increase ~3 and ~5 fold respectively in each culture condition. Two measures of motility, cell sheet area and migration distance, vary with amount of exogenous TGF-β1 and culture media. Addition of 100 ng/ml exogenous TGF-β1 in SFM increases both measures ((3.3 fold (p = 4.5 x 10-5) and 26% (p = 2.1 x 10-2) respectively)). In contrast, 100 ng/ml exogenous TGF-β1 into media containing 2% FBS decreases migration distance by 2.1 fold (p = 1.7 x 10-7) but does not affect sheet area. 10 ng/ml TGF-β1 has little effect on cell sheet area in SFM cultures but leads to a 1.8 fold increase (p = 1.5 x 10-2) with 2% FBS. The variable response to TGF-β1 may be, at least in part, explained by the effect of 2% FBS on cell morphology, mode of motility, and expression of endogenous TGF-β1 and TGFβRII. Together, these data suggest that expression of TGF-β and its receptor are up-regulated during zebrafish keratocyte explant culture and that TGF-β promotes fish keratocyte migration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping