PUBLICATION
Molecular Cloning and mRNA Expression of the Liver-Specific Cathepsin L1 Gene of the Olive Flounder, Paralichthys olivaceus
- Authors
- Kim, Y.O., Park, E.M., Seo, J.S., Nam, B.H., Kong, H.J., Kim, W.J., Kim, B.S., Kim, K.K., and Lee, S.J.
- ID
- ZDB-PUB-110628-17
- Date
- 2011
- Source
- Bioscience, biotechnology, and biochemistry 75(6): 1214-1218 (Journal)
- Registered Authors
- Keywords
- paralichthys olvaceus, cathepsin L, gene expression, RT-PCR, in situ hybridization
- MeSH Terms
-
- Amino Acid Motifs
- Animals
- Cathepsin L/genetics
- Cathepsin L/metabolism*
- Cloning, Molecular
- Escherichia coli
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Flounder/genetics
- Flounder/metabolism*
- Gene Expression
- Gene Library
- Humans
- Liver/metabolism*
- Molecular Sequence Data
- Phylogeny
- Plasmids
- RNA, Messenger
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Transformation, Bacterial
- PubMed
- 21670505 Full text @ Biosci. Biotechnol. Biochem.
Citation
Kim, Y.O., Park, E.M., Seo, J.S., Nam, B.H., Kong, H.J., Kim, W.J., Kim, B.S., Kim, K.K., and Lee, S.J. (2011) Molecular Cloning and mRNA Expression of the Liver-Specific Cathepsin L1 Gene of the Olive Flounder, Paralichthys olivaceus. Bioscience, biotechnology, and biochemistry. 75(6):1214-1218.
Abstract
We isolated a homolog of cathepsin L from a cDNA library of the olive flounder liver. The flounder cathepsin L1 transcript consisted of 1,221 bp that encoded a polypeptide of 334 amino acids. The overall identity between flounder cathepsin L1 and other cathepsin Ls was 50–64%, and flounder cathepsin L1 contained the highly conserved ERFNIN-motif. A phylogenetic tree indicated that flounder cathepsin L1 is in the same monophyletic group as zebrafish cathepsin Lc. RT-PCR analysis revealed that cathepsin L1 transcripts were expressed only in the liver. They were detected from 28 d post-hatching. Under starvation conditions, cathepsin L1 expression was decreased at 30 d.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping