PUBLICATION

Cloning, promoter analysis and expression in response to bacterial exposure of sea bass (Dicentrarchus labrax L.) interleukin-12 p40 and p35 subunits

Authors
Nascimento, D.S., do Vale, A., Tomás, A.M., Zou, J., Secombes, C.J., and dos Santos, N.M.
ID
ZDB-PUB-110621-1
Date
2007
Source
Molecular immunology   44(9): 2277-2291 (Journal)
Registered Authors
Keywords
sea bass, dicentrarchus labrax, IL-12, IL-12 p35, IL-12 p40, cytokines, gene expression, promoter
MeSH Terms
  • Bass/genetics*
  • Bass/microbiology*
  • Promoter Regions, Genetic/genetics*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Exons/genetics
  • Humans
  • Gene Expression Regulation*
  • Photobacterium/physiology*
  • Sequence Homology, Amino Acid
  • Introns/genetics
  • Animals
  • Molecular Sequence Data
  • Phylogeny
  • Cloning, Molecular
  • Tetraodontiformes/genetics
  • Interleukin-12 Subunit p40/chemistry
  • Interleukin-12 Subunit p40/genetics*
  • Interleukin-12 Subunit p40/metabolism
  • Amino Acid Sequence
  • Interleukin-12 Subunit p35/chemistry
  • Interleukin-12 Subunit p35/genetics*
  • Interleukin-12 Subunit p35/metabolism
(all 23)
PubMed
17196658 Full text @ Mol. Immunol.
Abstract
Interleukin-12 (IL-12) is a heterodimeric cytokine pivotal in resistance to microbial and viral infections. In the search for immunoregulatory genes in sea bass the genes for the two IL-12 subunits p40 and p35 were cloned and sequenced. Molecular characterization of these two genes was performed at both the cDNA and genomic levels. Sea bass IL-12 p40 and p35 conserve most cysteines involved in the intra-chain disulfide bonds of human IL-12 subunits as well as the important structural residues for human IL-12 heterodimerization. The gene organization of sea bass IL-12 p40 is similar to the human orthologue, whilst the sea bass IL-12 p35 gene structure, as reported for pufferfish, differs from the human one in containing an additional exon and lacking a second copy of a duplicated exon present in the mammalian genes. The promoter analysis of both sea bass and pufferfish IL-12 genes showed the presence of the main cis-acting elements involved in the transcriptional regulation of human and mouse orthologues. The involvement of IL-12 in sea bass anti-bacterial immune responses was demonstrated by investigating the expression profiles of IL-1beta, IL-12 p40 and p35 in the head-kidney and spleen following intraperitoneal injection of UV-killed and live Photobacterium damselae ssp. piscicida (Phdp). Finally, the importance of nuclear factor (NF)-kappaB on UV-killed Phdp-induced IL-12 p40 and p35 gene transcription was shown by the use of pyrrolidine dithiocarbamate (PDTC).
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Nascimento, D.S., do Vale, A., Tomás, A.M., Zou, J., Secombes, C.J., and dos Santos, N.M. (2007) Cloning, promoter analysis and expression in response to bacterial exposure of sea bass (Dicentrarchus labrax L.) interleukin-12 p40 and p35 subunits. Molecular immunology. 44(9):2277-2291.