PUBLICATION
Transplantation of cells directly into the kidney of adult zebrafish
- Authors
- Diep, C.Q., and Davidson, A.J.
- ID
- ZDB-PUB-110609-49
- Date
- 2011
- Source
- Journal of visualized experiments : JoVE (51): (Journal)
- Registered Authors
- Davidson, Alan
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Hematopoietic Stem Cell Transplantation/methods
- Hematopoietic Stem Cells/cytology
- Kidney/cytology
- Kidney/surgery*
- Male
- Regenerative Medicine/methods*
- Zebrafish
- PubMed
- 21633330 Full text @ J. Vis. Exp.
Citation
Diep, C.Q., and Davidson, A.J. (2011) Transplantation of cells directly into the kidney of adult zebrafish. Journal of visualized experiments : JoVE. (51).
Abstract
Regenerative medicine based on the transplantation of stem or progenitor cells into damaged tissues has the potential to treat a wide range of chronic diseases1. However, most organs are not easily accessible, necessitating the need to develop surgical methods to gain access to these structures. In this video article, we describe a method for transplanting cells directly into the kidney of adult zebrafish, a popular model to study regeneration and disease2. Recipient fish are pre-conditioned by irradiation to suppress the immune rejection of the injected cells3. We demonstrate how the head kidney can be exposed by a lateral incision in the flank of the fish, followed by the injection of cells directly in to the organ. Using fluorescently labeled whole kidney marrow cells comprising a mixed population of renal and hematopoietic precursors, we show that nephron progenitors can engraft and differentiate into new renal tissue - the gold standard of any cell-based regenerative therapy. This technique can be adapted to deliver purified stem or progenitor cells and/or small molecules to the kidney as well as other internal organs and further enhances the zebrafish as a versatile model to study regenerative medicine.
Errata / Notes
Video article + print.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping