PUBLICATION
The microcephaly gene aspm is involved in brain development in zebrafish
- Authors
- Kim, H.T., Lee, M.S., Choi, J.H., Jung, J.Y., Ahn, D.G., Yeo, S.Y., Choi, D.K., and Kim, C.H.
- ID
- ZDB-PUB-110609-13
- Date
- 2011
- Source
- Biochemical and Biophysical Research Communications 409(4): 640-4 (Journal)
- Registered Authors
- Ahn, Dae-gwon, Choi, Jung-Hwa, Kim, Cheol-Hee, Kim, Hyun-Taek, Lee, Mi-Sun, Yeo, Sang-Yeob
- Keywords
- Microcephaly; ASPM; CNS; Mitotic arrest; Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Brain/embryology*
- Cell Cycle
- Cell Cycle Proteins/genetics*
- Gene Knockdown Techniques
- Humans
- Microcephaly/genetics*
- Mitosis/genetics
- Molecular Sequence Data
- Organ Size/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics*
- PubMed
- 21620798 Full text @ Biochem. Biophys. Res. Commun.
Citation
Kim, H.T., Lee, M.S., Choi, J.H., Jung, J.Y., Ahn, D.G., Yeo, S.Y., Choi, D.K., and Kim, C.H. (2011) The microcephaly gene aspm is involved in brain development in zebrafish. Biochemical and Biophysical Research Communications. 409(4):640-4.
Abstract
MCPH is a neurodevelopmental disorder characterized by a global reduction in cerebral cortical volume. Homozygous mutation of the MCPH5 gene, also known as ASPM, is the most common cause of the MCPH phenotype. To elucidate the roles of ASPM during embryonic development, the zebrafish aspm was identified, which is specifically expressed in proliferating cells in the CNS. Morpholino-mediated knock-down of aspm resulted in a significant reduction in head size. Furthermore, aspm-deficient embryos exhibited a mitotic arrest during early development. These findings suggest that the reduction in brain size in MCPH might be caused by lack of aspm function in the mitotic cell cycle and demonstrate that the zebrafish can provide a model system for congenital diseases of the human nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping