PUBLICATION
Image-based fluidic sorting system for automated zebrafish egg sorting into multiwell plates
- Authors
- Graf, S.F., Hötzel, S., Liebel, U., Stemmer, A., and Knapp, H.F.
- ID
- ZDB-PUB-110603-10
- Date
- 2011
- Source
- Journal of Laboratory Automation 16(2): 105-111 (Journal)
- Registered Authors
- Liebel, Urban
- Keywords
- toxicity testing, cell sorting, automated, multiwell plate, zebrafish, danio rerio
- MeSH Terms
-
- Animals
- Hydrobiology/methods
- Organisms, Genetically Modified
- Ovum/classification*
- Reproducibility of Results
- Survival Analysis
- Toxicity Tests/methods
- Toxicology/methods
- Zebrafish*
- PubMed
- 21609691 Full text @ J. Lab. Autom.
Citation
Graf, S.F., Hötzel, S., Liebel, U., Stemmer, A., and Knapp, H.F. (2011) Image-based fluidic sorting system for automated zebrafish egg sorting into multiwell plates. Journal of Laboratory Automation. 16(2):105-111.
Abstract
The global demand for the reduction of animal testing has led to the emergence of Zebrafish eggs/larvae as model organisms to replace current adult animal testing in, for example, toxicity testing. Because of the egg size (diameter 1.6 mm) and the relatively easy maintenance of Zebrafish farms the eggs also offer high-throughput screening (HTS). However, the current bottleneck for HTS is the cost-efficient placing of individual organisms into single wells of a multiwell plate (MWP). The system presented here is capable of storing, sorting, and placing individual organisms in a highly reproducible manner. In about 11 min a complete 96-MWP is filled, which corresponds to about 8 sec per egg. The survival rate of fertilized transgenic and wild-type eggs was comparable to the one of the control (control 6.7%, system 7.6%). Furthermore, it was also possible to place dechorionated eggs into individual wells. The results demonstrate that the cost efficient system works gentle and reliable enough to disburden scientists from the exhausting and monotonous job of placing single eggs into single wells, such that they can concentrate on the scientific aspects of their experiments and create results with a higher statistical relevance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping