PUBLICATION
Global gene expression profile induced by the UV-filter 2-ethyl-hexyl-4-trimethoxycinnamate (EHMC) in zebrafish (Danio rerio)
- Authors
- Zucchi, S., Oggier, D.M., and Fent, K.
- ID
- ZDB-PUB-110602-32
- Date
- 2011
- Source
- Environmental pollution (Barking, Essex : 1987) 159(10): 3086-96 (Journal)
- Registered Authors
- Keywords
- UV-filter; EHMC; Danio rerio; Microarray; Transcriptional changes
- MeSH Terms
-
- Animals
- Cinnamates/toxicity*
- Gene Expression/drug effects*
- Male
- Sunscreening Agents/toxicity*
- Ultraviolet Rays
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 21601967 Full text @ Environ. Pollut.
Citation
Zucchi, S., Oggier, D.M., and Fent, K. (2011) Global gene expression profile induced by the UV-filter 2-ethyl-hexyl-4-trimethoxycinnamate (EHMC) in zebrafish (Danio rerio). Environmental pollution (Barking, Essex : 1987). 159(10):3086-96.
Abstract
Residues of the UV-filter 2-ethyl-hexyl-4-trimethoxycinnamate (EHMC) are ubiquitously found in aquatic biota but potential adverse effects in fish are fairly unknown. To identify molecular effects and modes of action of EHMC we applied a gene expression profiling in zebrafish using whole genome microarrays. Transcriptome analysis and validation of targeted genes were performed after 14 days of exposure of male zebrafish. Concentrations of 2.2 µg/L and 890 μg/L EHMC lead to alteration of 1096 and 1137 transcripts, respectively, belonging to many pathways. Genes involved in lipid metabolism and estrogenic pathway (vtg1), lipid biosynthesis (ptgds), vitamin A metabolic process (rbp2a), DNA damage and apoptosis (gadd45b), and regulation of cell growth (igfbp1a) were investigated by qRT-PCR analysis in whole body, liver, brain and testis. The analysis showed tissue-specific gene profiles and revealed that EHMC slightly affects the transcription of genes involved in hormonal pathways including vtg1, esr1, esr2b, ar, cyp19b and hsd17β3.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping