PUBLICATION

Chitosan coated carbon fiber microelectrode for selective in vivo detection of neurotransmitters in live zebrafish embryos

Authors
Ozel, R.E., Wallace, K.N., and Andreescu, S.
ID
ZDB-PUB-110602-31
Date
2011
Source
Analytica chimica acta   695(1-2): 89-95 (Journal)
Registered Authors
Wallace, Kenneth
Keywords
carbon fiber microelectrode, chitosan, ascorbic acid, interferences, in vivo detection of neurotransmitters, zebrafish, intestine
MeSH Terms
  • Animals
  • Carbon/chemistry*
  • Chitosan/chemistry*
  • Electrochemistry
  • Embryo, Nonmammalian/chemistry*
  • Microelectrodes
  • Neurotransmitter Agents/analysis*
  • Serotonin/analysis*
  • Zebrafish/embryology*
PubMed
21601035 Full text @ Anal. Chim. Acta
Abstract
We report the development of a chitosan modified carbon fiber microelectrode for in vivo detection of serotonin. We find that chitosan has the ability to reject physiological levels of ascorbic acid interferences and facilitate selective and sensitive detection of in vivo levels of serotonin, a common catecholamine neurotransmitter. Presence of chitosan on the microelectrode surface was investigated using scanning electron microscopy (SEM) and cyclic voltammetry (CV). The electrode was characterized using differential pulse voltammetry (DPV). A detection limit of 1.6nM serotonin with a sensitivity of 5.12nA/μM, a linear range from 2 to 100nM and a reproducibility of 6.5% for n=6 electrodes were obtained. Chitosan modified microelectrodes selectively measure serotonin in presence of physiological levels of ascorbic acid. In vivo measurements were performed to measure concentration of serotonin in the live embryonic zebrafish intestine. The sensor quantifies in vivo intestinal levels of serotonin while successfully rejecting ascorbic acid interferences. We demonstrate that chitosan can be used as an effective coating to reject ascorbic acid interferences at carbon fiber microelectrodes, as an alternative to Nafion, and that chitosan modified microelectrodes are reliable tools for in vivo monitoring of changes in neurotransmitter levels.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping