PUBLICATION
The Apoptotic Regulator Nrz Controls Cytoskeletal Dynamics via the Regulation of Ca(2)+ Trafficking in the Zebrafish Blastula
- Authors
- Popgeorgiev, N., Bonneau, B., Ferri, K.F., Prudent, J., Thibaut, J., and Gillet, G.
- ID
- ZDB-PUB-110524-33
- Date
- 2011
- Source
- Developmental Cell 20(5): 663-676 (Journal)
- Registered Authors
- Gillet, Germain
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis
- Blastula/cytology
- Blastula/metabolism*
- Calcium/metabolism*
- Cytoskeleton/metabolism*
- Endoplasmic Reticulum/metabolism
- HeLa Cells
- Humans
- Proto-Oncogene Proteins/metabolism*
- Zebrafish
- Zebrafish Proteins/metabolism*
- PubMed
- 21571223 Full text @ Dev. Cell
Citation
Popgeorgiev, N., Bonneau, B., Ferri, K.F., Prudent, J., Thibaut, J., and Gillet, G. (2011) The Apoptotic Regulator Nrz Controls Cytoskeletal Dynamics via the Regulation of Ca(2)+ Trafficking in the Zebrafish Blastula. Developmental Cell. 20(5):663-676.
Abstract
Bcl-2 family members are key regulators of apoptosis. Their involvement in other cellular processes has been so far overlooked. We have studied the role of the Bcl-2 homolog Nrz in the developing zebrafish. Nrz was found to be localized to the yolk syncytial layer, a region containing numerous mitochondria and ER membranes. Nrz knockdown resulted in developmental arrest before gastrulation, due to free Ca(2+) increase in the yolk cell, activating myosin light chain kinase, which led to premature contraction of actin-myosin cables in the margin and separation of the blastomeres from the yolk cell. In the yolk syncytial layer, Nrz appears to prevent the release of Ca(2+) from the endoplasmic reticulum by directly interacting with the IP3R1 Ca(2+) channel. Thus, the Bcl-2 family may participate in early development, not only by controlling apoptosis but also by acting on cytoskeletal dynamics and cell movements via Ca(2+) fluxes inside the embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping