PUBLICATION
Study of Pluripotency Markers in Zebrafish Embryos and Transient Embryonic Stem Cell Cultures
- Authors
- Robles, V., Martí, M., and Belmonte, J.C.
- ID
- ZDB-PUB-110524-16
- Date
- 2011
- Source
- Zebrafish 8(2): 57-63 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Biomarkers
- Cells, Cultured
- Embryonic Stem Cells/cytology*
- Embryonic Stem Cells/metabolism
- Female
- Gene Expression Regulation, Developmental*
- Male
- Pluripotent Stem Cells/cytology*
- Pluripotent Stem Cells/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 21563922 Full text @ Zebrafish
Citation
Robles, V., Martí, M., and Belmonte, J.C. (2011) Study of Pluripotency Markers in Zebrafish Embryos and Transient Embryonic Stem Cell Cultures. Zebrafish. 8(2):57-63.
Abstract
Abstract Targeted genomic manipulation using embryonic stem (ES) cells has not yet been achieved in zebrafish, although methods for zebrafish ES cell culture has been described in literature. The knowledge of pluripotency markers in this species is almost nonexistent and this is a very limiting factor in the definition of the ideal culture conditions for ES cells. Here, we studied the expression of several genes associated with pluripotency in zebrafish embryonic cells versus differentiated cells and the expression of some of these genes is recorded throughout embryonic development. Some of the commonly accepted pluripotency markers are also tested in embryonic cells, transient embryonic cell cultures, and differentiated cells. Our results support the hypothesis that stage-specific embryonic antigen 1 (SSEA1) is a marker that precedes the expression of pluripotency genes in a zebrafish embryonic cell colony, in the same way that SOX2 precedes nestin expression in those colonies that have already started differentiation toward neurons. We consider this study a step forward in the knowledge of zebrafish pluripotency markers and, therefore, an important tool for the monitoring of zebrafish embryonic cell cultures.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping