PUBLICATION

Endothelial Fate and Angiogenic Properties of Human CD34+ Progenitor Cells in Zebrafish

Authors
Pozzoli, O., Vella, P., Iaffaldano, G., Parente, V., Devanna, P., Lacovich, M., Lamia, C.L., Fascio, U., Longoni, D., Cotelli, F., Capogrossi, M.C., and Pesce, M.
ID
ZDB-PUB-110519-2
Date
2011
Source
Arterioscler. Thromb. Vasc. Biol.   31(7): 1589-97 (Journal)
Registered Authors
Cotelli, Franco
Keywords
endothelium, vascular biology, angiogenesis, embryology, stem cells
MeSH Terms
  • Cell Differentiation*/drug effects
  • Hematopoietic Stem Cells*/immunology
  • Neovascularization, Physiologic*
  • Paracrine Communication
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • RNA Interference
  • Amputation, Surgical
  • Animal Fins/blood supply*
  • Animal Fins/surgery
  • Animals, Genetically Modified
  • Endothelial Cells/immunology
  • Endothelial Cells/transplantation*
  • Animals
  • Phenotype
  • Hematopoietic Stem Cell Transplantation*
  • Cell Movement
  • Antigens, CD34/analysis*
  • Cord Blood Stem Cell Transplantation*
  • Regeneration
  • Fetal Blood/cytology*
  • Fetal Blood/immunology
  • Vascular Endothelial Growth Factor C/genetics
  • Vascular Endothelial Growth Factor C/metabolism
  • Recombinant Fusion Proteins/metabolism
  • Caco-2 Cells
  • Gene Expression Regulation, Developmental
  • Signal Transduction
  • Humans
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Zebrafish*/growth & development
(all 32)
PubMed
21527751 Full text @ Arterioscler. Thromb. Vasc. Biol.
Abstract
Objective? The vascular competence of human-derived hematopoietic progenitors for postnatal vascularization is still poorly characterized. It is unclear whether, in the absence of ischemia, hematopoietic progenitors participate in neovascularization and whether they play a role in new blood vessel formation by incorporating into developing vessels or by a paracrine action.

Methods and Results? In the present study, human cord blood?derived CD34+ (hCD34+) cells were transplanted into pre- and postgastrulation zebrafish embryos and in an adult vascular regeneration model induced by caudal fin amputation. When injected before gastrulation, hCD34+ cells cosegregated with the presumptive zebrafish hemangioblasts, characterized by Scl and Gata2 expression, in the anterior and posterior lateral mesoderm and were involved in early development of the embryonic vasculature. These morphogenetic events occurred without apparent lineage reprogramming, as shown by CD45 expression. When transplanted postgastrulation, hCD34+ cells were recruited into developing vessels, where they exhibited a potent paracrine proangiogenic action. Finally, hCD34+ cells rescued vascular defects induced by Vegf-c in vivo targeting and enhanced vascular repair in the zebrafish fin amputation model.

Conclusion? These results indicate an unexpected developmental ability of human-derived hematopoietic progenitors and support the hypothesis of an evolutionary conservation of molecular pathways involved in endothelial progenitor differentiation in vivo.

Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Pozzoli, O., Vella, P., Iaffaldano, G., Parente, V., Devanna, P., Lacovich, M., Lamia, C.L., Fascio, U., Longoni, D., Cotelli, F., Capogrossi, M.C., and Pesce, M. (2011) Endothelial Fate and Angiogenic Properties of Human CD34+ Progenitor Cells in Zebrafish. Arterioscler. Thromb. Vasc. Biol.. 31(7):1589-97.