PUBLICATION

Characterization of a mannose-binding lectin from channel catfish (Ictalurus punctatus)

Authors
Zhang, H., Peatman, E., Liu, H., Niu, D., Feng, T., Kucuktas, H., Waldbieser, G., Chen, L., and Liu, Z.
ID
ZDB-PUB-110518-48
Date
2012
Source
Research in Veterinary Science   92(3): 408-413 (Journal)
Registered Authors
Keywords
channel catfish, ictalurus punctalus, mannose-binding lectin, edwardsiella ictaluri, MBL, lectin
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Brain/metabolism
  • Cloning, Molecular
  • Edwardsiella ictaluri
  • Enterobacteriaceae Infections/immunology
  • Enterobacteriaceae Infections/metabolism
  • Enterobacteriaceae Infections/veterinary
  • Gene Expression Regulation/immunology
  • Head Kidney/metabolism
  • Ictaluridae/metabolism*
  • Kidney/metabolism
  • Liver/metabolism
  • Mannose-Binding Lectin/chemistry*
  • Mannose-Binding Lectin/genetics
  • Mannose-Binding Lectin/metabolism*
  • Molecular Sequence Data
  • Muscle, Skeletal/metabolism
  • Phylogeny
  • Protein Binding
  • Protein Structure, Tertiary
  • Reverse Transcriptase Polymerase Chain Reaction/veterinary
  • Skin/metabolism
  • Spleen/metabolism
  • Stomach/metabolism
PubMed
21524427 Full text @ Res. Vet. Sci.
Abstract
Mannose-binding lectin (MBL) is an important component of innate immunity capable of activating the lectin pathway of the complement system. A MBL gene was isolated from channel catfish (Ictalurus punctatus). The deduced protein contains a canonical collagen-like domain, a carbohydrate recognition domain (CRD), and a neck region similar to mammalian mannose-binding lectin. The catfish mannose-binding lectin CRD contains the EPN motif shown previously to mediate mannose specificity. The catfish mannose-binding lectin showed 30-43% identity with MBL protein sequences of rainbow trout, zebrafish, common carp, and goldfish, and 33-35% identity with sequences of mammalian species. In this study, while liver was the predominant source of mannose-binding lectin gene expression in healthy tissues, mannose-binding lectin expression in spleen rose sharply following challenge with a Gram-negative bacterium.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping