PUBLICATION
Characterization of a mannose-binding lectin from channel catfish (Ictalurus punctatus)
- Authors
- Zhang, H., Peatman, E., Liu, H., Niu, D., Feng, T., Kucuktas, H., Waldbieser, G., Chen, L., and Liu, Z.
- ID
- ZDB-PUB-110518-48
- Date
- 2012
- Source
- Research in Veterinary Science 92(3): 408-413 (Journal)
- Registered Authors
- Keywords
- channel catfish, ictalurus punctalus, mannose-binding lectin, edwardsiella ictaluri, MBL, lectin
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Brain/metabolism
- Cloning, Molecular
- Edwardsiella ictaluri
- Enterobacteriaceae Infections/immunology
- Enterobacteriaceae Infections/metabolism
- Enterobacteriaceae Infections/veterinary
- Gene Expression Regulation/immunology
- Head Kidney/metabolism
- Ictaluridae/metabolism*
- Kidney/metabolism
- Liver/metabolism
- Mannose-Binding Lectin/chemistry*
- Mannose-Binding Lectin/genetics
- Mannose-Binding Lectin/metabolism*
- Molecular Sequence Data
- Muscle, Skeletal/metabolism
- Phylogeny
- Protein Binding
- Protein Structure, Tertiary
- Reverse Transcriptase Polymerase Chain Reaction/veterinary
- Skin/metabolism
- Spleen/metabolism
- Stomach/metabolism
- PubMed
- 21524427 Full text @ Res. Vet. Sci.
Citation
Zhang, H., Peatman, E., Liu, H., Niu, D., Feng, T., Kucuktas, H., Waldbieser, G., Chen, L., and Liu, Z. (2012) Characterization of a mannose-binding lectin from channel catfish (Ictalurus punctatus). Research in Veterinary Science. 92(3):408-413.
Abstract
Mannose-binding lectin (MBL) is an important component of innate immunity capable of activating the lectin pathway of the complement system. A MBL gene was isolated from channel catfish (Ictalurus punctatus). The deduced protein contains a canonical collagen-like domain, a carbohydrate recognition domain (CRD), and a neck region similar to mammalian mannose-binding lectin. The catfish mannose-binding lectin CRD contains the EPN motif shown previously to mediate mannose specificity. The catfish mannose-binding lectin showed 30-43% identity with MBL protein sequences of rainbow trout, zebrafish, common carp, and goldfish, and 33-35% identity with sequences of mammalian species. In this study, while liver was the predominant source of mannose-binding lectin gene expression in healthy tissues, mannose-binding lectin expression in spleen rose sharply following challenge with a Gram-negative bacterium.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping