Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence
- Authors
- Matsuda, Y., Ito, Y., Hashimoto, H., Yokoi, H., and Suzuki, T.
- ID
- ZDB-PUB-110512-15
- Date
- 2011
- Source
- Reproductive Biology and Endocrinolgy 9: 45 (Journal)
- Registered Authors
- Hashimoto, Hisashi, Yokoi, Hayato
- Keywords
- none
- MeSH Terms
-
- Goldfish/metabolism*
- Fluorescein-5-isothiocyanate/analysis
- Animals
- Microscopy, Fluorescence/methods*
- Lymph/metabolism
- Zebrafish/metabolism*
- Estrogens/pharmacology
- Vitellogenins/analysis
- Vitellogenins/metabolism*
- Female
- PubMed
- 21481233 Full text @ Reprod. Biol. Endocrinol.
BACKGROUND:
Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.
METHODS:
Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.
RESULTS:
Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.
CONCLUSION:
This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.