ZFIN ID: ZDB-PUB-110512-15
Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence
Matsuda, Y., Ito, Y., Hashimoto, H., Yokoi, H., and Suzuki, T.
Date: 2011
Source: Reproductive Biology and Endocrinolgy   9: 45 (Journal)
Registered Authors: Hashimoto, Hisashi, Yokoi, Hayato
Keywords: none
MeSH Terms:
  • Animals
  • Estrogens/pharmacology
  • Female
  • Fluorescein-5-isothiocyanate/analysis
  • Goldfish/metabolism*
  • Lymph/metabolism
  • Microscopy, Fluorescence/methods*
  • Vitellogenins/analysis
  • Vitellogenins/metabolism*
  • Zebrafish/metabolism*
PubMed: 21481233 Full text @ Reprod. Biol. Endocrinol.
FIGURES
ABSTRACT

BACKGROUND:

Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.

METHODS:

Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.

RESULTS:

Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.

CONCLUSION:

This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.

ADDITIONAL INFORMATION No data available