ZFIN ID: ZDB-PUB-110413-4
A highly conserved cis-regulatory motif directs differential gonadal synexpression of Dmrt1 transcripts during gonad development
Herpin, A., Nakamura, S., Wagner, T.U., Tanaka, M., and Schartl, M.
Date: 2009
Source: Nucleic acids research 37(5): 1510-1520 (Journal)
Registered Authors: Schartl, Manfred, Tanaka, Minoru, Wagner, Toni
Keywords: none
MeSH Terms:
  • 3' Untranslated Regions/chemistry*
  • Animals
  • Cells, Cultured
  • Fish Proteins/biosynthesis
  • Fish Proteins/genetics*
  • Gene Expression Regulation, Developmental*
  • Gonads/embryology
  • Gonads/growth & development
  • Gonads/metabolism*
  • Humans
  • Mesoderm/metabolism
  • Oryzias/embryology
  • Oryzias/genetics
  • Oryzias/growth & development
  • Protein Biosynthesis
  • RNA Stability
  • RNA, Messenger/metabolism
  • RNA-Binding Proteins/metabolism
  • Regulatory Sequences, Ribonucleic Acid*
  • Transcription Factors/biosynthesis
  • Transcription Factors/genetics*
PubMed: 19139075 Full text @ Nucleic Acids Res.
ABSTRACT
Differential gene expression largely accounts for the coordinated manifestation of the genetic programme underlying embryonic development and cell differentiation. The 3' untranslated region (3'-UTR) of eukaryotic genes can contain motifs involved in regulation of gene expression at the post-transcriptional level. In the 3'-UTR of dmrt1, a key gene that functions in gonad development and differentiation, an 11-bp protein-binding motif was identified that mediates gonad-specific mRNA localization during embryonic and larval development of fish. Mutations that disrupt the 11-bp motif leading to in vitro protein-binding loss and selective transcript stabilization failure indicate a role for this motif in RNA stabilization through protein binding. The sequence motif was found to be conserved in most of the dmrt1 homologous genes from flies to humans suggesting a widespread conservation of this specific mechanism.
ADDITIONAL INFORMATION