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ZFIN ID: ZDB-PUB-110317-40
Behavioral and physiological effects of RDX on adult zebrafish
Williams, L.R., Wong, K., Stewart, A., Suciu, C., Gaikwad, S., Wu, N., Dileo, J., Grossman, L., Cachat, J., Hart, P., and Kalueff, A.V.
Date: 2012
Source: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 155(1): 33-8 (Journal)
Registered Authors: Cachat, Jonathan, Kalueff, Allan V.
Keywords: RDX, Seizures, Epilepsy, Pro-convulsant action, Behavioral activation, Cortisol, Zebrafish
MeSH Terms:
  • Animals
  • Behavior, Animal/drug effects*
  • Female
  • Hydrocortisone/analysis
  • Hyperkinesis/chemically induced
  • Locomotion/drug effects
  • Male
  • Seizures/chemically induced
  • Spasm/chemically induced
  • Swimming
  • Time Factors
  • Toxicity Tests, Acute
  • Triazines/toxicity*
  • Zebrafish/physiology*
PubMed: 21382508 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
ABSTRACT
1,3,5-Trinitroperhydro-1,3,5-triazine (RDX) is a nitroamine explosive, with common toxic effects including seizures. Here, we explore the behavioral effects of acute RDX exposure in adult zebrafish Danio rerio, a rapidly developing model in neuroscience and neurotoxicology research. Overall, a 30-min exposure to RDX low dose of 0.1 mM evoked behavioral activation in zebrafish, while a higher dose of 1 mM markedly reduced exploration, increased freezing and evoked seizure-like responses (i.e., bouts of hyperactivity, spasms, and corkscrew swimming). Likewise, whole-body cortisol levels were also significantly elevated in fish exposed to 1 mM (but not 0.1 mM) RDX. In line with clinical and animal data, our study demonstrates the dose-dependent behavioral activation and pro-convulsant effects of RDX in zebrafish-based models.
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