PUBLICATION
Cloning and characterization of TIMP-2b gene in grass carp
- Authors
- Xu, X.Y., Shen, Y.B., Yang, X.M., and Li, J.L.
- ID
- ZDB-PUB-110317-32
- Date
- 2011
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 159(2): 115-21 (Journal)
- Registered Authors
- Keywords
- Grass carp, TIMP-2b, Expression
- MeSH Terms
-
- Aeromonas hydrophila/growth & development*
- Amino Acid Sequence
- Animals
- Carps/embryology
- Carps/genetics
- Carps/metabolism*
- Carps/microbiology
- Cloning, Molecular
- DNA, Complementary/analysis
- Embryonic Development/genetics
- Escherichia coli
- Female
- Fish Diseases/genetics
- Fish Diseases/metabolism*
- Fish Diseases/microbiology
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Gene Expression Regulation, Developmental
- Gram-Negative Bacterial Infections/genetics
- Gram-Negative Bacterial Infections/metabolism*
- Gram-Negative Bacterial Infections/microbiology
- Matrix Metalloproteinase Inhibitors
- Matrix Metalloproteinases/genetics
- Matrix Metalloproteinases/metabolism
- Molecular Sequence Data
- Ovum/growth & development
- Ovum/metabolism
- Phylogeny
- RNA, Messenger/analysis
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Sequence Homology, Amino Acid
- Spleen/chemistry
- Spleen/metabolism
- Tissue Inhibitor of Metalloproteinase-2/genetics
- Tissue Inhibitor of Metalloproteinase-2/metabolism*
- PubMed
- 21377537 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Xu, X.Y., Shen, Y.B., Yang, X.M., and Li, J.L. (2011) Cloning and characterization of TIMP-2b gene in grass carp. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 159(2):115-21.
Abstract
Tissue inhibitors of metalloproteinases (TIMPs) are the primary inhibitors of matrix metalloproteinases (MMPs) in tissues. In this study, a TIMP-2 gene was isolated from grass carp Ctenopharyngodon idella (CiTIMP-2b) and characterized. This cDNA sequence encoded a signal peptide, N- and C-terminal domains. CiTIMP-2b is highly homologous to the orthologous in zebrafish and other teleosts, suggesting TIMP-2b is highly conserved during teleost evolution. CiTIMP-2b gene is expressed in a wide range of tissues including blood, brain, muscle, trunk kidney, liver, head kidney, skin, spleen, heart, gill, intestine and fin, with the highest level of transcripts in spleen. Upon challenge with Aeromonas hydrophila, its expression was significantly up-regulated in all tissues. The CiTIMP-2b transcript is present at unfertilized eggs, which suggests that CiTIMP-2b transcript is maternally inherited. These results suggest that the CiTIMP-2b would play an important role in the A. hydrophila-related diseases and early embryonic development stages in grass carp.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping