PUBLICATION
Genotoxic damages in zebrafish submitted to a polymetallic gradient displayed by the Lot River (France)
- Authors
- Orieux, N., Cambier, S., Gonzalez, P., Morin, B., Adam, C., Garnier-Laplace, J., and Bourdineaud, J.P.
- ID
- ZDB-PUB-110221-4
- Date
- 2011
- Source
- Ecotoxicology and environmental safety 74(4): 974-983 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Zebrafish, Cadmium, Zinc, Bioaccumulation, Genotoxicity, RAPD, Ecotoxicology, Genomic DNA, Quantitative PCR
- MeSH Terms
-
- Animals
- Cadmium/analysis
- Cadmium/metabolism
- Cadmium/toxicity
- Comet Assay
- DNA Damage
- DNA Repair
- Deoxyguanosine/analogs & derivatives
- Deoxyguanosine/metabolism
- Environmental Monitoring
- France
- Gene Expression/drug effects
- Mutagens/analysis
- Mutagens/metabolism
- Mutagens/toxicity*
- Random Amplified Polymorphic DNA Technique
- Rivers/chemistry*
- Water Pollutants, Chemical/analysis
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism*
- Zinc/analysis
- Zinc/metabolism
- Zinc/toxicity
- PubMed
- 21315451 Full text @ Ecotoxicol. Environ. Saf.
- CTD
- 21315451
Citation
Orieux, N., Cambier, S., Gonzalez, P., Morin, B., Adam, C., Garnier-Laplace, J., and Bourdineaud, J.P. (2011) Genotoxic damages in zebrafish submitted to a polymetallic gradient displayed by the Lot River (France). Ecotoxicology and environmental safety. 74(4):974-983.
Abstract
Genotoxic effects of a polymetallic pollution gradient displayed by the Lot River and one of its tributary have been assessed on zebrafish Danio rerio. Three methods were compared: RAPD-PCR, the comet assay, and 8-oxo-7, 8-dihydro-2′-deoxyguanosine (8-oxodG) formation. The fishes were exposed for 14 days to waters collected from three stations: Joanis, a site polluted by cadmium (Cd) and zinc (Zn) (mean concentrations: 15μg Cd/L and 550μg Zn/L), Bouillac (mean concentrations: 0.55μg Cd/L and 80μg Zn/L), and Boisse-Penchot, a reference station (mean concentrations:<0.05μg Cd/L and 7μg Zn/L). The quantitative RAPD-PCR methodology proved to be sensitive enough to unmask metal genotoxicity after 3 and 7 days of exposure to Joanis water and after 14 days to Bouillac water, whereas the comet assay only detected DNA damages at the most contaminated station (Joanis). The 8-oxodG quantification was not sensitive enough to be used in zebrafish under these environmental conditions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping