PUBLICATION
Wnt/?-catenin dependent cell proliferation underlies segmented lateral line morphogenesis
- Authors
- Aman, A., Nguyen, M., and Piotrowski, T.
- ID
- ZDB-PUB-110218-3
- Date
- 2011
- Source
- Developmental Biology 349(2): 470-482 (Journal)
- Registered Authors
- Aman, Andy, Nguyen, Minh Tu, Piotrowski, Tatjana
- Keywords
- Segmentation, Lateral line, Morphogenesis
- MeSH Terms
-
- Animals
- Cell Proliferation*/drug effects
- Transcription Factors/metabolism
- In Situ Hybridization
- Hydroxyurea
- Signal Transduction/drug effects
- Signal Transduction/physiology*
- Gene Knockout Techniques
- Pyrroles/pharmacology
- Fibroblast Growth Factors/metabolism
- Wnt Proteins/metabolism*
- Bromodeoxyuridine
- Aphidicolin
- Embryo, Nonmammalian/embryology
- beta Catenin/metabolism*
- Lateral Line System/embryology*
- Morphogenesis/physiology*
- DNA Primers/genetics
- Time-Lapse Imaging
- In Situ Nick-End Labeling
- Zebrafish/embryology*
- PubMed
- 20974120 Full text @ Dev. Biol.
Citation
Aman, A., Nguyen, M., and Piotrowski, T. (2011) Wnt/?-catenin dependent cell proliferation underlies segmented lateral line morphogenesis. Developmental Biology. 349(2):470-482.
Abstract
Morphogenesis is a fascinating but complex and incompletely understood developmental process. The sensory lateral line system consists of only a few hundred cells and is experimentally accessible making it an excellent model system to interrogate the cellular and molecular mechanisms underlying segmental morphogenesis. The posterior lateral line primordium periodically deposits prosensory organs as it migrates to the tail tip. We demonstrate that periodic proneuromast deposition is governed by a fundamentally different developmental mechanism than the classical models of developmental periodicity represented by vertebrate somitogenesis and early Drosophila development. Our analysis demonstrates that proneuromast deposition is driven by periodic lengthening of the primordium and a stable Wnt/β-catenin activation domain in the leading region of the primordium. The periodic lengthening of the primordium is controlled by Wnt/β-catenin/Fgf-dependent proliferation. Once proneuromasts are displaced into the trailing Wnt/β-catenin-free zone they are deposited. We have previously shown that Wnt/β-catenin signaling induces Fgf signaling and that interactions between these two pathways regulate primordium migration and prosensory organ formation. Therefore, by coordinating migration, prosensory organ formation and proliferation, localized activation of Wnt/β-catenin signaling in the leading zone of the primordium plays a crucial role in orchestrating lateral line morphogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping