PUBLICATION
Zebrafish HSC70 promoter to express carp muscle-specific creatine kinase for acclimation under cold condition
- Authors
- Wu, C.L., Lin, T.H., Chang, T.L., Sun, H.W., Hui, C.F., and Wu, J.L.
- ID
- ZDB-PUB-110214-13
- Date
- 2011
- Source
- Transgenic Research 20(6): 1217-26 (Journal)
- Registered Authors
- Sun, His-Wen, Wu, Jen-Leih
- Keywords
- Creatine kinase, Heat shock cognate, Hsc 70, Cold, Inducible promoter, Zebrafish
- MeSH Terms
-
- Acclimatization*
- Animals
- Animals, Genetically Modified
- Carps/genetics*
- Cold Temperature
- Creatine Kinase/metabolism*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/physiology
- Enzyme Activation
- Escherichia coli/genetics
- Escherichia coli/metabolism
- Gene Expression Regulation
- HSC70 Heat-Shock Proteins/genetics*
- HSC70 Heat-Shock Proteins/metabolism
- Microinjections
- Models, Animal
- Plasmids/genetics
- Plasmids/metabolism
- Promoter Regions, Genetic*
- Swimming/physiology
- Transfection
- Transgenes
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish/physiology
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 21298478 Full text @ Transgenic. Res.
Citation
Wu, C.L., Lin, T.H., Chang, T.L., Sun, H.W., Hui, C.F., and Wu, J.L. (2011) Zebrafish HSC70 promoter to express carp muscle-specific creatine kinase for acclimation under cold condition. Transgenic Research. 20(6):1217-26.
Abstract
Zebrafish (Danio rerio) is used as a model system for in vivo studies. To expand the research scope of physical, biochemical and physiological studies, a cold-tolerant model of zebrafish was developed. The common carp (Cyprinus carpio) muscle form of creatine kinase (CK, EC 2.7.3.2) can maintain enzymatic activity at a temperature of around 15°C. However, a cold-inducible promoter of zebrafish, hsc 70 (heat shock protein 70 cognate), is able to increase the expression of gene product by 9.8 fold at a temperature of 16°C. Therefore, the carp CK gene was promoted by hsc 70 and transfected into zebrafish embryos. Resulting transgenic zebrafish survived and could maintain its swimming behavior at 13°C, which was not possible with the wild-type zebrafish. The swimming distance of the transgenic fish was 42% greater than that of the wild type at 13°C. This new transgenic fish model is ideal for studies of ectothermal vertebrates in low-temperature environments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping