PUBLICATION
Global Gene Expression Profiling in Larval Zebrafish Exposed to Microcystin-LR and Microcystis Reveals Endocrine Disrupting Effects of Cyanobacteria
- Authors
- Rogers, E.D., Henry, T.B., Twiner, M.J., Gouffon, J.S., McPherson, J.T., Boyer, G.L., Sayler, G.S., and Wilhelm, S.W.
- ID
- ZDB-PUB-110207-12
- Date
- 2011
- Source
- Environmental science & technology 45(5): 1962-1969 (Journal)
- Registered Authors
- Henry, Theodore B.
- Keywords
- none
- Datasets
- GEO:GSE12214
- MeSH Terms
-
- Animals
- Endocrine Disruptors/metabolism
- Endocrine Disruptors/toxicity*
- Gene Expression/drug effects*
- Larva/drug effects
- Microcystins/metabolism
- Microcystins/toxicity*
- Microcystis/growth & development
- Microcystis/metabolism
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 21280650 Full text @ Env. Sci. Tech.
Citation
Rogers, E.D., Henry, T.B., Twiner, M.J., Gouffon, J.S., McPherson, J.T., Boyer, G.L., Sayler, G.S., and Wilhelm, S.W. (2011) Global Gene Expression Profiling in Larval Zebrafish Exposed to Microcystin-LR and Microcystis Reveals Endocrine Disrupting Effects of Cyanobacteria. Environmental science & technology. 45(5):1962-1969.
Abstract
Microcystis blooms occur worldwide and threaten aquatic ecosystems and human health. Sublethal effects on early developmental stages of fish are largely unknown, and research has mainly focused on microcystin toxins (such as MC-LR) rather than Microcystis cells. We exposed (96 h) zebrafish larvae to purified MC-LR (0-1000 μg/L) or lyophilized Microcystis aeruginosa containing 4.5 μg/L MC-LR and evaluated changes in global gene expression (Affymetrix GeneChip zebrafish genome arrays). Significant changes in gene expression (≥1.7-fold change, p < 0.0001) were determined with Rosetta Resolver 7.0, and ontology analysis was conducted with the DAVID bioinformatics tool. The number of differentially expressed genes relative to control increased with MC-LR concentration and included genes related to known mechanisms of action for MC-LR in mammals and older life stages of fish, as well as genes unique to larval zebrafish. Up-regulation of vitellogenin genes (vtg) (19.2-fold to >100-fold on arrays; 619.3-fold confirmed by quantitative PCR) was observed in Microcystis -exposed larvae but not in larvae exposed to MC-LR. Up-regulation of vtg indicates exposure to estrogenic substance(s) and suggests that Microcystis may be a natural source of environmental estrogens. Concerns about effects of Microcystis blooms may extend beyond those associated with the microcystin toxin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping