PUBLICATION
Structures of SAS-6 Suggest Its Organization in Centrioles
- Authors
- van Breugel, M., Hirono, M., Andreeva, A., Yanagisawa, H.A., Yamaguchi, S., Nakazawa, Y., Morgner, N., Petrovich, M., Ebong, I.O., Robinson, C.V., Johnson, C.M., Veprintsev, D., and Zuber, B.
- ID
- ZDB-PUB-110131-37
- Date
- 2011
- Source
- Science (New York, N.Y.) 331(6021): 1196-1199 (Journal)
- Registered Authors
- Johnson, Christopher
- Keywords
- none
- MeSH Terms
-
- Protein Multimerization
- Recombinant Proteins/chemistry
- Recombinant Proteins/metabolism
- Cell Cycle Proteins/chemistry
- Cell Cycle Proteins/metabolism
- Amino Acid Motifs
- Chromosomal Proteins, Non-Histone/chemistry*
- Chromosomal Proteins, Non-Histone/metabolism
- Zebrafish
- Humans
- Centrosome/metabolism
- Protein Structure, Quaternary
- Centrioles/chemistry*
- Centrioles/metabolism
- Centrioles/ultrastructure
- Models, Molecular
- Hydrophobic and Hydrophilic Interactions
- Flagella/metabolism
- Flagella/ultrastructure
- Animals
- Chlamydomonas reinhardtii/chemistry
- Chlamydomonas reinhardtii/metabolism
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/metabolism
- Point Mutation
- Mutant Proteins/chemistry
- Crystallography, X-Ray
- Protein Structure, Tertiary
- Cell Line, Tumor
- PubMed
- 21273447 Full text @ Science
Citation
van Breugel, M., Hirono, M., Andreeva, A., Yanagisawa, H.A., Yamaguchi, S., Nakazawa, Y., Morgner, N., Petrovich, M., Ebong, I.O., Robinson, C.V., Johnson, C.M., Veprintsev, D., and Zuber, B. (2011) Structures of SAS-6 Suggest Its Organization in Centrioles. Science (New York, N.Y.). 331(6021):1196-1199.
Abstract
Centrioles are cylindrical, nine-fold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the N-terminal domain of SAS-6 from zebrafish and show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are likely essential to the structural organization of cartwheel centers.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping