ZFIN ID: ZDB-PUB-110131-31
Bisphenol A induces otolith malformations during vertebrate embryogenesis
Gibert, Y., Sassi-Messai, S., Fini, J.B., Bernard, L., Zalko, D., Cravedi, J.P., Balaguer, P., Andersson-Lendahl, M., Demeneix, B., and Laudet, V.
Date: 2011
Source: BMC Developmental Biology   11(1): 4 (Journal)
Registered Authors: Gibert, Yann, Laudet, Vincent, Lendahl, Monika Andersson
Keywords: Bisphenol A, Endocrine disruptors, Toxicology, Zebrafish, Embryo, Otic vesicle, Otolith
MeSH Terms:
  • Animals
  • Benzhydryl Compounds/metabolism
  • Embryonic Development/drug effects*
  • Endocrine Disruptors/toxicity*
  • Environmental Pollution
  • Estradiol/analogs & derivatives
  • Estradiol/pharmacology
  • Female
  • In Situ Hybridization
  • Otolithic Membrane/abnormalities
  • Otolithic Membrane/embryology*
  • Otolithic Membrane/physiology
  • Ouabain/pharmacology
  • Phenols/metabolism
  • Phenols/toxicity*
  • Receptors, Estrogen/metabolism
  • Receptors, Thyroid Hormone
  • Sodium-Potassium-Exchanging ATPase/metabolism*
  • Thyroid Hormones/pharmacology
  • Water Pollutants
  • Xenopus
  • Zebrafish
PubMed: 21269433 Full text @ BMC Dev. Biol.
BACKGROUND: The plastic monomer and plasticizer bisphenol A (BPA), used for manufacturing polycarbonate plastic and epoxy resins, is produced at over 2.5 million metric tons per year. Concerns have been raised that BPA acts as an endocrine disruptor on both developmental and reproductive processes and a large body of evidence suggests that BPA interferes with estrogen and thyroid hormone signaling. Here, we investigated BPA effects during embryonic development using the zebrafish and Xenopus models.
RESULTS: We report that BPA exposure leads to severe malformations of the otic vesicle. In zebrafish and in Xenopus embryos, exposure to BPA during the first developmental day resulted in dose-dependent defects in otolith formation. Defects included aggregation, multiplication and occasionally failure to form otoliths. As no effects on otolith development were seen with exposure to micromolar concentrations of thyroid hormone, 17-beta-estradiol or of the estrogen receptor antagonist ICI 182,780 we conclude that the effects of BPA are independent of estrogen receptors or thyroid-hormone receptors. Na+/K+ ATPases are crucial for otolith formation in zebrafish. Pharmacological inhibition of the major Na+/K+ ATPase with ouabain can rescue the BPA-induced otolith phenotype.
CONCLUSIONS: The data suggest that the spectrum of BPA action is wider than previously expected and argue for a systematic survey of the developmental effects of this endocrine disruptor.