PUBLICATION

Bisphenol A induces otolith malformations during vertebrate embryogenesis

Authors
Gibert, Y., Sassi-Messai, S., Fini, J.B., Bernard, L., Zalko, D., Cravedi, J.P., Balaguer, P., Andersson-Lendahl, M., Demeneix, B., and Laudet, V.
ID
ZDB-PUB-110131-31
Date
2011
Source
BMC Developmental Biology   11(1): 4 (Journal)
Registered Authors
Gibert, Yann, Laudet, Vincent, Lendahl, Monika Andersson
Keywords
Bisphenol A, Endocrine disruptors, Toxicology, Zebrafish, Embryo, Otic vesicle, Otolith
MeSH Terms
  • Zebrafish
  • Xenopus
  • Animals
  • Estradiol/analogs & derivatives
  • Estradiol/pharmacology
  • Female
  • Receptors, Estrogen/metabolism
  • Ouabain/pharmacology
  • Benzhydryl Compounds/metabolism
  • Receptors, Thyroid Hormone
  • Embryonic Development/drug effects*
  • Thyroid Hormones/pharmacology
  • Water Pollutants
  • In Situ Hybridization
  • Phenols/metabolism
  • Phenols/toxicity*
  • Environmental Pollution
  • Otolithic Membrane/abnormalities
  • Otolithic Membrane/embryology*
  • Otolithic Membrane/physiology
  • Endocrine Disruptors/toxicity*
  • Sodium-Potassium-Exchanging ATPase/metabolism*
(all 22)
PubMed
21269433 Full text @ BMC Dev. Biol.
CTD
21269433
Abstract
BACKGROUND: The plastic monomer and plasticizer bisphenol A (BPA), used for manufacturing polycarbonate plastic and epoxy resins, is produced at over 2.5 million metric tons per year. Concerns have been raised that BPA acts as an endocrine disruptor on both developmental and reproductive processes and a large body of evidence suggests that BPA interferes with estrogen and thyroid hormone signaling. Here, we investigated BPA effects during embryonic development using the zebrafish and Xenopus models.
RESULTS: We report that BPA exposure leads to severe malformations of the otic vesicle. In zebrafish and in Xenopus embryos, exposure to BPA during the first developmental day resulted in dose-dependent defects in otolith formation. Defects included aggregation, multiplication and occasionally failure to form otoliths. As no effects on otolith development were seen with exposure to micromolar concentrations of thyroid hormone, 17-beta-estradiol or of the estrogen receptor antagonist ICI 182,780 we conclude that the effects of BPA are independent of estrogen receptors or thyroid-hormone receptors. Na+/K+ ATPases are crucial for otolith formation in zebrafish. Pharmacological inhibition of the major Na+/K+ ATPase with ouabain can rescue the BPA-induced otolith phenotype.
CONCLUSIONS: The data suggest that the spectrum of BPA action is wider than previously expected and argue for a systematic survey of the developmental effects of this endocrine disruptor.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf233TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    atp1a1a.1MO2-atp1a1a.1MRPHLNO
    oc90MO1-oc90MRPHLNO
    1 - 2 of 2
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    Fish
    Fish
    zf233Tg
    1 - 1 of 1
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    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    LuciferaseEFGLuciferase
    1 - 1 of 1
    Show
    Mapping
    No data available
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    Citation
    Gibert, Y., Sassi-Messai, S., Fini, J.B., Bernard, L., Zalko, D., Cravedi, J.P., Balaguer, P., Andersson-Lendahl, M., Demeneix, B., and Laudet, V. (2011) Bisphenol A induces otolith malformations during vertebrate embryogenesis. BMC Developmental Biology. 11(1):4.