PUBLICATION

Embryonic mesoderm and endoderm induction requires the actions of non-embryonic Nodal-related ligands and Mxtx2

Authors
Hong, S.K., Jang, M.K., Brown, J.L., McBride, A.A., and Feldman, B.
ID
ZDB-PUB-110131-24
Date
2011
Source
Development (Cambridge, England)   138(4): 787-795 (Journal)
Registered Authors
Feldman, Benjamin, Hong, Sung-Kook
Keywords
Mxtx2, Ndr1, Ndr2, Ntla, Mesendoderm, Yolk syncytial layer, Zebrafish
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/metabolism*
  • Endoderm/metabolism*
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/metabolism*
  • Ligands
  • Mesoderm/metabolism*
  • Nodal Signaling Ligands/genetics
  • Nodal Signaling Ligands/metabolism*
  • Transcription, Genetic
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 18)
PubMed
21266414 Full text @ Development
Abstract
Vertebrate mesoderm and endoderm formation requires signaling by Nodal-related ligands from the TGFβ superfamily. The factors that initiate Nodal-related gene transcription are unknown in most species and the relative contributions of Nodal-related ligands from embryonic, extraembryonic and maternal sources remain uncertain. In zebrafish, signals from the yolk syncytial layer (YSL), an extraembryonic domain, are required for mesoderm and endoderm induction, and YSL expression of nodal-related 1 (ndr1) and ndr2 accounts for a portion of this activity. A variable requirement of maternally derived Ndr1 for dorsal and anterior axis formation has also been documented. Here we show that Mxtx2 directly activates expression of ndr2 via binding to its first intron and is required for ndr2 expression in the YSL. Mxtx2 is also required for the Nodal signaling-independent expression component of the no tail a (ntla) gene, which is required for posterior (tail) mesoderm formation. Therefore, Mxtx2 defines a new pathway upstream of Nodal signaling and posterior mesoderm formation. We further show that the co-disruption of extraembryonic Ndr2, extraembryonic Ndr1 and maternal Ndr1 eliminates endoderm and anterior (head and trunk) mesoderm, recapitulating the loss of Nodal signaling phenotype. Therefore, non-embryonic sources of Nodal-related ligands account for the complete spectrum of early Nodal signaling requirements. In summary, the induction of mesoderm and endoderm depends upon the combined actions of Mxtx2 and Nodal-related ligands from non-embryonic sources.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hi975TgTransgenic Insertion
tz257
    		Point Mutation
    1 - 2 of 2
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    mxtx2MO1-mxtx2MRPHLNO
    ndr1MO2-ndr1MRPHLNO
    ndr1MO4-ndr1MRPHLNO
    ndr2MO1-ndr2MRPHLNO
    1 - 4 of 4
    Show
    Fish
    Fish
    ndr1hi975Tg/hi975Tg
    ndr1hi975Tg/hi975Tg
    ndr1hi975Tg/hi975Tg
    tdgf1tz257/tz257
    1 - 4 of 4
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    Hong, S.K., Jang, M.K., Brown, J.L., McBride, A.A., and Feldman, B. (2011) Embryonic mesoderm and endoderm induction requires the actions of non-embryonic Nodal-related ligands and Mxtx2. Development (Cambridge, England). 138(4):787-795.