PUBLICATION
Global proteomics analysis of testis and ovary in adult zebrafish (Danio rerio)
- Authors
- Groh, K.J., Nesatyy, V.J., Segner, H., Eggen, R.I., and Suter, M.J.
- ID
- ZDB-PUB-110119-30
- Date
- 2011
- Source
- Fish physiology and biochemistry 37(3): 619-47 (Journal)
- Registered Authors
- Keywords
- Sex differentiation, Testis, Ovary, Proteomics, Multidimensional protein identification technology (MudPIT), Zebrafish
- MeSH Terms
-
- Animals
- Apoptosis
- Cell Cycle Proteins/genetics
- Cell Cycle Proteins/metabolism
- Cyclin-Dependent Kinases/genetics
- Cyclin-Dependent Kinases/metabolism
- Cytokines/genetics
- Cytokines/metabolism
- DNA/genetics
- DNA/metabolism
- Dyneins/genetics
- Dyneins/metabolism
- Egg Proteins/genetics
- Egg Proteins/metabolism
- Female
- Gene Expression Regulation/physiology
- Intercellular Signaling Peptides and Proteins/genetics
- Intercellular Signaling Peptides and Proteins/metabolism
- Male
- Membrane Glycoproteins/genetics
- Membrane Glycoproteins/metabolism
- Ovary/metabolism*
- Phosphorylation
- Proteomics*
- RNA/genetics
- RNA/metabolism
- Receptors, Cell Surface/genetics
- Receptors, Cell Surface/metabolism
- SOX Transcription Factors/genetics
- SOX Transcription Factors/metabolism
- Septins/genetics
- Septins/metabolism
- Sex Characteristics
- Signal Transduction/physiology
- Testis/metabolism*
- Vitellogenins/genetics
- Vitellogenins/metabolism
- Zebrafish/metabolism*
- PubMed
- 21229308 Full text @ Fish Physiol. Biochem.
Citation
Groh, K.J., Nesatyy, V.J., Segner, H., Eggen, R.I., and Suter, M.J. (2011) Global proteomics analysis of testis and ovary in adult zebrafish (Danio rerio). Fish physiology and biochemistry. 37(3):619-47.
Abstract
The molecular mechanisms controlling sex determination and differentiation in zebrafish (Danio rerio) are largely unknown. A genome-wide analysis may provide comprehensive insights into the processes involved. The mRNA expression in zebrafish gonads has been fairly well studied, but much less data on the corresponding protein expression are available, although the proteins are considered to be more relevant markers of gene function. Because mRNA and protein abundances rarely correlate well, mRNA profiles need to be complemented with the information on protein expression. The work presented here analyzed the proteomes of adult zebrafish gonads by a multidimensional protein identification technology, generating the to-date most populated lists of proteins expressed in mature zebrafish gonads. The acquired proteomics data partially confirmed existing transcriptomics information for several genes, including several novel transcripts. However, disagreements between mRNA and protein abundances were often observed, further stressing the necessity to assess the expression on different levels before drawing conclusions on a certain gene's expression and function. Several gene groups expressed in a sexually dimorphic way in zebrafish gonads were identified. Their potential importance for gonad development and function is discussed. The data gained in the current study provide a basis for further work on elucidating processes occurring during zebrafish development with use of high-throughput proteomics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping