PUBLICATION
Efficient mutation identification in zebrafish by microarray capturing and next generation sequencing
- Authors
- Bontems, F., Baerlocher, L., Mehenni, S., Bahechar, I., Farinelli, L., and Dosch, R.
- ID
- ZDB-PUB-110119-14
- Date
- 2011
- Source
- Biochemical and Biophysical Research Communications 405(3): 373-376 (Journal)
- Registered Authors
- Bontems, Franck, Dosch, Roland
- Keywords
- Zebrafish, Maternal effect mutation, Embryonic polarity, Embryogenesis, Genomics, Microarray capturing, Next generation sequencing, Microtubule actin crosslinking factor 1 (macf1)
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- DNA Mutational Analysis/methods*
- Female
- Male
- Molecular Sequence Data
- Mutation
- Oligonucleotide Array Sequence Analysis/methods*
- Sequence Deletion
- Zebrafish/genetics*
- PubMed
- 21219859 Full text @ Biochem. Biophys. Res. Commun.
Citation
Bontems, F., Baerlocher, L., Mehenni, S., Bahechar, I., Farinelli, L., and Dosch, R. (2011) Efficient mutation identification in zebrafish by microarray capturing and next generation sequencing. Biochemical and Biophysical Research Communications. 405(3):373-376.
Abstract
Fish models like Medaka, stickleback or zebrafish provide a valuable resource to study vertebrate genes. However, finding genetic variants e.g. mutations in the genome is still arduous. Here we used a combination of microarray capturing and next generation sequencing to identify the affected gene in the mozartkugel(p11cv) (mzl(p11cv)) mutant zebrafish. We discovered a 31-bp deletion in macf1 demonstrating the potential of this technique to efficiently isolate mutations in a vertebrate genome.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping