PUBLICATION
Multicolor brainbow imaging in zebrafish
- Authors
- Pan, Y.A., Livet, J., Sanes, J.R., Lichtman, J.W., and Schier, A.F.
- ID
- ZDB-PUB-110110-29
- Date
- 2011
- Source
- Cold Spring Harbor protocols 2011: pdb.prot5546 (Journal)
- Registered Authors
- Pan, Y. Albert, Schier, Alexander
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/cytology
- Image Processing, Computer-Assisted/methods*
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism*
- Neurons/cytology
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Staining and Labeling/methods*
- Zebrafish/anatomy & histology*
- PubMed
- 21205846 Full text @ Cold Spring Harb. Protoc.
Citation
Pan, Y.A., Livet, J., Sanes, J.R., Lichtman, J.W., and Schier, A.F. (2011) Multicolor brainbow imaging in zebrafish. Cold Spring Harbor protocols. 2011:pdb.prot5546.
Abstract
This protocol describes how to use the Brainbow strategy to label neurons in many different hues. The Brainbow system uses a random Cre/lox recombination to create varied combinations of red, blue, and green fluorescent proteins in each cell. The differences in color allow users to follow multiple cells, regardless of how closely they are positioned. This protocol describes how to use Brainbow imaging in zebrafish and provides examples of how to use color as a guide to trace axonal processes. We use the zebrafish trigeminal sensory ganglion as an example and discuss potential modifications for the general use of this technique.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping