PUBLICATION

Identification and expression patterns of members of the protease-activated receptor (par) gene family during zebrafish development

Authors
Xu, H., Echemendia, N., Chen, S., and Lin, F.
ID
ZDB-PUB-110103-12
Date
2011
Source
Developmental dynamics : an official publication of the American Association of Anatomists   240(1): 278-287 (Journal)
Registered Authors
Lin, Fang, Xu, Hui
Keywords
protease-activated receptors (PARs), expression pattern, zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Embryo, Nonmammalian
  • Embryonic Development/genetics
  • Gene Expression Profiling
  • Molecular Sequence Data
  • Multigene Family/genetics
  • Phylogeny
  • Protein Transport/genetics
  • Receptor, PAR-1/genetics*
  • Receptor, PAR-1/isolation & purification
  • Receptor, PAR-1/metabolism
  • Receptor, PAR-2/genetics
  • Receptor, PAR-2/metabolism
  • Receptors, Thrombin/genetics*
  • Receptors, Thrombin/isolation & purification
  • Receptors, Thrombin/metabolism
  • Sequence Homology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
21181945 Full text @ Dev. Dyn.
Abstract
Protease-activated receptors (PARs) play critical roles in hemostasis in vertebrates including zebrafish. However, the zebrafish gene classification appears to be complex, and the expression patterns of par genes are not established. Based on analyses of genomic organization, phylogenetics, protein primary structure, and protein internalization, we report the identification of four zebrafish PARs: par1, par2a, par2b, and par3. This classification differs from one reported previously. We also show that these genes have distinct spatiotemporal expression profiles in embryos and larvae, with par1, par2a, and par2b expressed maternally and ubiquitously during gastrula stages and their expression patterns refined at later stages, and par3 expressed only in 3-day-old larvae. Notably, the expression patterns of zebrafish par1 and par2b resemble those of their mammalian counterparts, suggesting that receptor function is conserved among vertebrates. This conservation is supported by our findings that Par1 and Par2b are internalized following exposure to thrombin and trypsin, respectively.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes