PUBLICATION
Hypoxia-induced metastasis model in embryonic zebrafish
- Authors
- Rouhi, P., Jensen, L.D., Cao, Z., Hosaka, K., Länne, T., Wahlberg, E., Steffensen, J.F., and Cao, Y.
- ID
- ZDB-PUB-101209-16
- Date
- 2010
- Source
- Nature Protocols 5(12): 1911-1918 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line, Tumor
- Disease Models, Animal*
- Embryo, Nonmammalian
- Humans
- Hypoxia/complications*
- Mice
- Microscopy, Fluorescence
- Neoplasm Invasiveness/physiopathology
- Neoplasm Metastasis/pathology*
- Neovascularization, Pathologic/etiology
- Neovascularization, Pathologic/pathology*
- Zebrafish/embryology*
- PubMed
- 21127485 Full text @ Nat. Protoc.
Citation
Rouhi, P., Jensen, L.D., Cao, Z., Hosaka, K., Länne, T., Wahlberg, E., Steffensen, J.F., and Cao, Y. (2010) Hypoxia-induced metastasis model in embryonic zebrafish. Nature Protocols. 5(12):1911-1918.
Abstract
Hypoxia facilitates tumor invasion and metastasis by promoting neovascularization and co-option of tumor cells in the peritumoral vasculature, leading to dissemination of tumor cells into the circulation. However, until recently, animal models and imaging technology did not enable monitoring of the early events of tumor cell invasion and dissemination in living animals. We recently developed a zebrafish metastasis model to dissect the detailed events of hypoxia-induced tumor cell invasion and metastasis in association with angiogenesis at the single-cell level. In this model, fluorescent DiI-labeled human or mouse tumor cells are implanted into the perivitelline cavity of 48-h-old zebrafish embryos, which are subsequently placed in hypoxic water for 3 d. Tumor cell invasion, metastasis and pathological angiogenesis are detected under fluorescent microscopy in the living fish. The average experimental time for this model is 7 d. Our protocol offers a remarkable opportunity to study molecular mechanisms of hypoxia-induced cancer metastasis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping