PUBLICATION
Hypoxia-induced retinopathy model in adult zebrafish
- Authors
- Cao, Z., Jensen, L.D., Rouhi, P., Hosaka, K., Länne, T., Steffensen, J.F., Wahlberg, E., and Cao, Y.
- ID
- ZDB-PUB-101209-15
- Date
- 2010
- Source
- Nature Protocols 5(12): 1903-1910 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Diabetic Retinopathy/etiology*
- Disease Models, Animal*
- Green Fluorescent Proteins/metabolism
- Hypoxia/complications*
- Microscopy, Fluorescence
- Neovascularization, Pathologic/etiology
- Neovascularization, Pathologic/pathology*
- Zebrafish*
- PubMed
- 21127484 Full text @ Nat. Protoc.
Citation
Cao, Z., Jensen, L.D., Rouhi, P., Hosaka, K., Länne, T., Steffensen, J.F., Wahlberg, E., and Cao, Y. (2010) Hypoxia-induced retinopathy model in adult zebrafish. Nature Protocols. 5(12):1903-1910.
Abstract
Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1:EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping