ZFIN ID: ZDB-PUB-101209-13
Hedgehog signalling is required for perichondral osteoblast differentiation in zebrafish
Felber, K., Croucher, P., and Roehl, H.H.
Date: 2011
Source: Mechanisms of Development   128(1-2): 141-152 (Journal)
Registered Authors: Roehl, Henry
Keywords: Dermal bone, Perichondrium, Periosteum, Hedgehog, Osteoblast osteoprogenetor, Osteoblast, Osteoblastogenesis, Neural crest, Bone, Cranial, runx2/cbfa1/osf1/pebp2αA, Osterix
MeSH Terms:
  • Animals
  • Biomarkers/metabolism
  • Cell Differentiation*/drug effects
  • Chondrocytes/cytology
  • Chondrocytes/drug effects
  • Chondrocytes/metabolism
  • Collagen Type X/metabolism
  • Down-Regulation/drug effects
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental/drug effects
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/metabolism*
  • Osteoblasts/cytology
  • Osteoblasts/drug effects
  • Osteoblasts/metabolism
  • Osteogenesis/drug effects
  • Receptors, Cell Surface/deficiency
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism*
  • Signal Transduction*/drug effects
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Veratrum Alkaloids/pharmacology
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 21126582 Full text @ Mech. Dev.
In tetrapod long bones, Hedgehog signalling is required for osteoblast differentiation in the perichondrium. In this work we analyse skeletogenesis in zebrafish larvae treated with the Hedgehog signalling inhibitor cyclopamine. We show that cyclopamine treatment leads to the loss of perichondral ossification of two bones in the head. We find that the Hedgehog co-receptors patched 1 and patched 2 are expressed in regions of the perichondrium that will form bone before the onset of ossification. We also show that cyclopamine treatment strongly reduces the expression of osteoblast markers in the perichondrium and that perichondral ossification is enhanced in patched 1 mutant fish. This data suggests a conserved role for Hedgehog signalling in promoting perichondral osteoblast differentiation during vertebrate skeletal development. However, unlike what is seen during long bone development, we did not observe ectopic chondrocytes in the perichondrium when Hedgehog signalling is blocked. This result may point to subtle differences between the development of the skeleton in the skull and limb.