PUBLICATION
Chapter 9 - Zebrafish kidney development
- Authors
- Drummond, I.A., and Davidson, A.J.
- ID
- ZDB-PUB-101201-31
- Date
- 2010
- Source
- Methods in cell biology 100: 233-260 (Chapter)
- Registered Authors
- Davidson, Alan, Drummond, Iain
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/physiology
- Kidney/cytology*
- Kidney/embryology*
- Kidney/physiology
- Zebrafish/embryology*
- Zebrafish/physiology
- PubMed
- 21111220 Full text @ Meth. Cell. Biol.
Citation
Drummond, I.A., and Davidson, A.J. (2010) Chapter 9 - Zebrafish kidney development. Methods in cell biology. 100:233-260.
Abstract
The zebrafish pronephric kidney provides a useful and relevant model of kidney development and function. It is composed of cell types common to all vertebrate kidneys and pronephric organogenesis is regulated by transcription factors that have highly conserved functions in mammalian kidney development. Pronephric nephrons are a good model of tubule segmentation and differentiation of epithelial cell types. The pronephric glomerulus provides a simple model to assay gene function in regulating cell structure and cell interactions that form the blood filtration apparatus. The relative simplicity of the pronephric kidney combined with the ease of genetic manipulation in zebrafish makes it well suited for mutation analysis and gene discovery, in vivo imaging, functional screens of candidate genes from other species, and cell isolation by FACS . In addition, the larval and adult zebrafish kidneys have emerged as systems to study kidney regeneration after injury. This chapter provides a review of pronephric structure and development as well as current methods to study the pronephros.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping