ZFIN ID: ZDB-PUB-101201-26
Chapter 4 - Neurogenesis
Chapouton, P., and Godinho, L.
Date: 2010
Source: Methods in cell biology   100: 72-126 (Chapter)
Registered Authors: Chapouton, Prisca, Godinho, Leanne
Keywords: none
MeSH Terms:
  • Animals
  • Brain/cytology
  • Neurobiology/methods*
  • Neurogenesis*
  • Zebrafish*
PubMed: 21111215 Full text @ Meth. Cell. Biol.
For more than a decade, the zebrafish has proven to be an excellent model organism to investigate the mechanisms of neurogenesis during development. The often cited advantages, namely external development, genetic, and optical accessibility, have permitted direct examination and experimental manipulations of neurogenesis during development. Recent studies have begun to investigate adult neurogenesis, taking advantage of its widespread occurrence in the mature zebrafish brain to investigate the mechanisms underlying neural stem cell maintenance and recruitment. Here we provide a comprehensive overview of the tools and techniques available to study neurogenesis in zebrafish both during development and in adulthood. As useful resources, we provide tables of available molecular markers, transgenic, and mutant lines. We further provide optimized protocols for studying neurogenesis in the adult zebrafish brain, including in situ hybridization, immunohistochemistry, in vivo lipofection and electroporation methods to deliver expression constructs, administration of bromodeoxyuridine (BrdU), and finally slice cultures. These currently available tools have put zebrafish on par with other model organisms used to investigate neurogenesis.