PUBLICATION
Isotocin controls ion regulation through regulating ionocyte progenitor differentiation and proliferation
- Authors
- Chou, M.Y., Hung, J.C., Wu, L.C., Hwang, S.P., and Hwang, P.P.
- ID
- ZDB-PUB-101201-14
- Date
- 2011
- Source
- Cellular and molecular life sciences : CMLS 68(16): 2797-809 (Journal)
- Registered Authors
- Chou, Ming-Yi, Hwang, Pung Pung, Hwang, Sheng-Ping L.
- Keywords
- Isotocin, Ionocyte, Zebrafish, Ion, Differentiation
- MeSH Terms
-
- Embryo, Nonmammalian/metabolism
- Animals
- Forkhead Transcription Factors/genetics
- Forkhead Transcription Factors/metabolism
- Trans-Activators/metabolism
- Stem Cells/cytology*
- Stem Cells/metabolism
- Ion Transport
- Ions/metabolism*
- Down-Regulation
- Cell Differentiation
- Oxytocin/analogs & derivatives*
- Oxytocin/genetics
- Oxytocin/metabolism
- Oxytocin/physiology
- Cell Proliferation
- Phosphoproteins/metabolism
- Zebrafish/embryology
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish Proteins/physiology
- PubMed
- 21104292 Full text @ Cell. Mol. Life Sci.
Citation
Chou, M.Y., Hung, J.C., Wu, L.C., Hwang, S.P., and Hwang, P.P. (2011) Isotocin controls ion regulation through regulating ionocyte progenitor differentiation and proliferation. Cellular and molecular life sciences : CMLS. 68(16):2797-809.
Abstract
The present study using zebrafish as a model explores the role of isotocin, a homolog of oxytocin, in controlling ion regulatory mechanisms. Double-deionized water treatment for 24 h significantly stimulated isotocin mRNA expression in zebrafish embryos. Whole-body Cl(-), Ca(2+), and Na(+) contents, mRNA expressions of ion transporters and ionocyte-differentiation related transcription factors, and the number of skin ionocytes decreased in isotocin morphants. In contrast, overexpression of isotocin caused an increase in ionocyte numbers. Isotocin morpholino caused significant suppression of foxi3a mRNA expression, while isotocin cRNA stimulated foxi3a mRNA expressions at the tail-bud stage of zebrafish embryos. The density of P63 (an epidermal stem cell marker)-positive cells was downregulated by isotocin morpholinos and was upregulated by isotocin cRNA. Taken together, isotocin stimulates the proliferation of epidermal stem cells and differentiation of ionocyte progenitors by regulating the P63 and Foxi3a transcription factors, consequently enhancing the functional activities of ionocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping