Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures
- Zhang, X., Ji, W., Zhang, H., Zhang, W., and Xie, P.
- Journal of applied toxicology : JAT 31(6): 561-7 (Journal)
- Registered Authors
- Ji, Wei, Zhang, Wei
- microcystin-LR, temperature, LD50, glutathione S-transferases, real-time PCR, zebrafish
- MeSH Terms
- Glutathione Transferase/genetics
- Glutathione Transferase/metabolism
- Lethal Dose 50
- Oxidative Stress/drug effects
- Toxicity Tests
- 21089159 Full text @ J. Appl. Toxicol.
Zhang, X., Ji, W., Zhang, H., Zhang, W., and Xie, P. (2011) Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures. Journal of applied toxicology : JAT. 31(6):561-7.
It is well known that fish have stronger tolerance than mammals to microcystin (MC) exposure, and such a difference is attributed to their different core body temperatures. However, no in vivo study has been conducted to investigate the effects of temperature on MC-induced toxicity in fish, a typical poikilotherm. Tolerance and detoxification response of zebrafish treated with MC-LR were investigated under three temperatures. The LD(50) values evidently increased with a decline of the temperature (547, 260 and 176 µg kg(-1) at 12, 22 and 32 °C, respectively), indicating stronger tolerance of the fish at lower temperatures. Changes in the transcription of glutathione S-transferase (GST) isoforms in the fish were observed, and their sensitivity of response in the transcription of GST mRNA was on the order of 12 > 32 > 22°C. We screened out several GST genes which were more delicate to solve the MC-LR exposure at different temperatures, i.e. GST rho1, al, p1 and theta1 in the 12 °C group, and GST zeta1 and p2 in the 22 and 32 °C groups. Our findings partly validate the hypothesis that high temperature enhances toxic effects of MCs on poikilotherms. Our studies also indicate that temperature-dependent toxic effects should be taken into account for field toxic assessment of microcystins in fish.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes