PUBLICATION
Construction and Characterization of the BAC Library for Common Carp Cyprinus Carpio L. And Establishment of Microsynteny with Zebrafish Danio Rerio
- Authors
- Li, Y., Xu, P., Zhao, Z., Wang, J., Zhang, Y., and Sun, X.W.
- ID
- ZDB-PUB-101122-27
- Date
- 2011
- Source
- Marine biotechnology (New York, N.Y.) 13(4): 706-12 (Journal)
- Registered Authors
- Wang, Jian
- Keywords
- BAC, Common carp, Genome, Comparative mapping, Synteny
- MeSH Terms
-
- Animals
- Base Sequence
- Carps/genetics*
- Chromosomes, Artificial, Bacterial*
- DNA Primers/genetics
- Gene Library*
- Genomics/methods
- Molecular Sequence Data
- Sequence Analysis, DNA
- Species Specificity
- Synteny/genetics*
- Zebrafish/genetics*
- PubMed
- 21088980 Full text @ Mar. Biotechnol.
Citation
Li, Y., Xu, P., Zhao, Z., Wang, J., Zhang, Y., and Sun, X.W. (2011) Construction and Characterization of the BAC Library for Common Carp Cyprinus Carpio L. And Establishment of Microsynteny with Zebrafish Danio Rerio. Marine biotechnology (New York, N.Y.). 13(4):706-12.
Abstract
A bacterial artificial chromosome (BAC) library of common carp Cyprinus carpio L. was constructed as a part of ongoing common carp genome project, which is aiming assembly of common carp genome. The library, containing a total of 92,160 BAC clones with an average insert size of 141 kb, was constructed into the restriction site of Hind III on BAC vector CopyControl pCC1BAC, covering 7.7 X haploid genome equivalents. Three dimension pools and superpools of the BAC library were established and 23 positive clones of 14 targets were identified from one-fifth of the BAC library. Pilot project of BAC end sequencing was conducted on 2,688 BAC ends from 1,344 clones and harvested 2,522 high-quality Q20 sequences with average length of 677 bp. The sequencing success rate was 93.8% and pair-end success rate was 92.3%. A total of 212 microsyntenies had been established between common carp and zebrafish genomes as a trial for genome-wide comparative genomics in these two closely related species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping