PUBLICATION
mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish
- Authors
- Ellett, F., Pase, L., Hayman, J.W., Andrianopoulos, A., and Lieschke, G.J.
- ID
- ZDB-PUB-101122-22
- Date
- 2011
- Source
- Blood 117(4): e49-e56 (Journal)
- Registered Authors
- Ellett, Felix, Hayman, John W., Lieschke, Graham J., Pase, Luke
- Keywords
- none
- MeSH Terms
-
- Animals, Genetically Modified
- Transgenes/physiology*
- Macrophages/metabolism*
- Macrophages/physiology
- Animals
- Promoter Regions, Genetic*
- Membrane Proteins/genetics
- Cell Lineage/genetics*
- Myeloid Cells/metabolism
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Cloning, Molecular
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Organ Specificity/genetics
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental
- PubMed
- 21084707 Full text @ Blood
Citation
Ellett, F., Pase, L., Hayman, J.W., Andrianopoulos, A., and Lieschke, G.J. (2011) mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish. Blood. 117(4):e49-e56.
Abstract
Macrophages and neutrophils play important roles during the innate immune response, phagocytosing invading microbes and delivering antimicrobial compounds to the site of injury. Functional analyses of the cellular innate immune response in zebrafish infection/inflammation models have been aided by transgenic lines with fluorophore-marked neutrophils. However, it has not been possible to study macrophage behaviors and neutrophil/macrophage interactions in vivo directly because there has been no macrophage-only reporter line. To remove this roadblock, a macrophage-specific marker was identified (mpeg1) and its promoter used in mpeg1-driven transgenes. mpeg1-driven transgenes are expressed in macrophage-lineage cells that do not express neutrophil-marking transgenes. Using these lines, the different dynamic behaviors of neutrophils and macrophages following wounding were compared side-by-side in compound transgenics. Macrophage/neutrophil interactions, such as phagocytosis of senescent neutrophils were readily observed in real time. These zebrafish transgenes provide a new resource that will contribute to the fields of inflammation, infection and leukocyte biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping