PUBLICATION
atp2b1a regulates Ca(2+) export during differentiation and regeneration of mechanosensory hair cells in zebrafish
- Authors
- Go, W., Bessarab, D., and Korzh, V.
- ID
- ZDB-PUB-101122-20
- Date
- 2010
- Source
- Cell Calcium 48(5): 302-313 (Journal)
- Registered Authors
- Bessarab, Dmitry, Go, William, Korzh, Vladimir
- Keywords
- Zebrafish, SqET4, PMCA1, Cell division, Transient progenitor
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Basic Helix-Loop-Helix Transcription Factors/physiology
- Calcium/metabolism*
- Cell Differentiation/physiology
- Gene Expression Regulation
- Gene Knockdown Techniques
- Green Fluorescent Proteins/biosynthesis
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Lateral Line System/cytology
- Lateral Line System/metabolism
- Lateral Line System/physiology*
- Mechanoreceptors/cytology
- Mechanoreceptors/metabolism
- Mechanoreceptors/physiology*
- Plasma Membrane Calcium-Transporting ATPases/biosynthesis
- Plasma Membrane Calcium-Transporting ATPases/genetics
- Plasma Membrane Calcium-Transporting ATPases/physiology*
- Regeneration
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 21084119 Full text @ Cell Calcium
Citation
Go, W., Bessarab, D., and Korzh, V. (2010) atp2b1a regulates Ca(2+) export during differentiation and regeneration of mechanosensory hair cells in zebrafish. Cell Calcium. 48(5):302-313.
Abstract
The molecular mechanisms of development of mechanosensory hair cells have been tackled successfully due to in vivo studies in the zebrafish lateral line. The enhancer trap (ET) transgenic line, SqET4 was instrumental in these studies even despite a lack of a link of its GFP expression pattern to a particular gene(s). We mapped the Tol2 transposon insertion of the SqET4 transgenics onto Chr. 4 next to a gene encoding Atp2b1a (Pmca1) - one of the four PMCAs acting to export Ca(2+) from a cell. atp2b1a expression recapitulates that of GFP during the development of mechanoreceptors of the inner ear and lateral line. atp2b1a expression correlates with the regeneration of these cells. Thus, SqET4 represents the Tg:atp2b1a-GFP line, which links Ca(2+) metabolism and the differentiation of mechanoreceptors. The morpholino-mediated knockdown of atp2b1a blocks Ca(2+) export and affects the division of hair cell progenitors, resulting in their accumulation. Under the control of a master gene of hair cells, Atoh1a, Atp2b1a functions during progenitor cell proliferation and hair cell differentiation. Given the similarity between the phenotypes of atp2b1a morphants and embryos treated with the pan-PMCA inhibitor 5(6)-carboxyeosin, Atp2b1a emerges as member of the Atp2b family responsible for Ca(2+) export during the development of hair cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping