ZFIN ID: ZDB-PUB-101115-13
Expression of ventral diencephalon-enriched genes in zebrafish
Li, S., Yin, M., Liu, S., Chen, Y., Yin, Y., Liu, T., and Zhou, J.
Date: 2010
Source: Developmental dynamics : an official publication of the American Association of Anatomists   239(12): 3368-3379 (Journal)
Registered Authors: Chen, Yi
Keywords: dopaminergic neuron, diencephalon, Id3, maturation
MeSH Terms:
  • Animals
  • Diencephalon/embryology
  • Diencephalon/metabolism*
  • Dopamine/metabolism*
  • Embryo, Nonmammalian/metabolism
  • Fatty Acid-Binding Proteins/genetics
  • Fatty Acid-Binding Proteins/metabolism
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • In Situ Hybridization
  • Inhibitor of Differentiation Proteins/genetics
  • Inhibitor of Differentiation Proteins/metabolism
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 21061380 Full text @ Dev. Dyn.
Dopaminergic (DA) neurons in the vertebrate di- and mesencephalon play essential roles in movement control, endocrine modulation and many other important physiological activities. To identify genes that may regulate the specification and differentiation of diencephalic DA neurons in zebrafish, the spatial and temporal expression pattern of a set of genes was investigated. In situ hybridization analysis revealed that expression of DNA binding inhibitor 3 (Id3), early B cell factor 2 (Ebf2), Ebf3, Iroquois related homeobox 1 (Irx1), Kruppel-like factor 7 (Klf7), mab-21-like 1 (Mab21l1), fatty acid binding protein 7 (Fabp7) and stathmin-like 4 (Stmn4), were enriched in the diencephalon of zebrafish. Among these genes, Id3 was expressed specifically in a subset of DA neurons in the ventral diencephalon, with co-expression of neurogenin1 (Ngn1). Alteration of expression levels of Id3 inhibited maturation of developing DA neurons. Taken together, our study provides genetic characteristics of DA neurons in the diencephalon of zebrafish.