PUBLICATION

The Role Of Meis1 In Primitive And Definitive Hematopoiesis During Zebrafish Development

Authors
Cvejic, A., Serbanovic-Canic, J., Stemple, D.L., and Ouwehand, W.H.
ID
ZDB-PUB-101108-31
Date
2011
Source
Haematologica   96(2): 190-198 (Journal)
Registered Authors
Stemple, Derek L.
Keywords
none
MeSH Terms
  • Animals
  • Cell Differentiation
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism*
  • Erythrocytes/cytology*
  • Erythrocytes/metabolism
  • Gene Expression Regulation, Developmental*
  • Hematopoiesis/physiology*
  • Homeodomain Proteins/physiology*
  • Immunoenzyme Techniques
  • In Situ Hybridization, Fluorescence
  • Macrophages/metabolism
  • Neoplasm Proteins/physiology*
  • Neutrophils/metabolism
  • Transcription Factors/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
PubMed
21048033 Full text @ Haematologica
Abstract
The Meis1 protein represents an important cofactor for Hox and Pbx1 and is implicated in human and murine leukaemias. Though much is known about the role of meis1 in leukaemogenesis, its function in normal haematopoiesis, remains largely unclear. Here we characterise the role of the proto-oncogene, meis1, during zebrafish primitive and definitive hematopoiesis. Design and Methods. Using antisense morpholino oligonucleotides to interrupt meis1 expression we find that, although primitive macrophage development can occur unhampered, the posterior erythroid differentiation requires meis1, resulting in severe decrease in number of mature erythrocytes. Furthermore a picture emerges that meis1 exerts important effects on later stages of erythrocyte maturation and these effects are independent of gata1, but under the control of scl. Results. In addition, meis1 morpholino knock-down leads to dramatic single arteriovenous tube formation. We also find that knock-down of pbx1 results in a strikingly similar phenotype to that of meis1 knock-down. Conclusion. Thus, these results implicate that meis1, jointly with pbx1, regulates primitive haematopoiesis as well as vascular development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping