PUBLICATION
Identification of zRAP55,a gene preponderantly expressed in Stages I and II oocytes of zebrafish
- Authors
- Zhao, C.L., Yang, Q.W., Hu, J.R., Ye, D., Gong, W.M., Lv, H.Y., Xu, Z.Y., Zhang, X.P., and Song, P.
- ID
- ZDB-PUB-101101-6
- Date
- 2010
- Source
- Dong wu xue yan jiu = Zoological research 31(5): 469-475 (Journal)
- Registered Authors
- Keywords
- Lsm domain, zRAP55, Oogenesis, Immunohistochemistry, Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Female
- Humans
- Male
- Molecular Sequence Data
- Oocytes/metabolism*
- RNA, Messenger/analysis
- RNA-Binding Proteins/analysis
- RNA-Binding Proteins/chemistry
- RNA-Binding Proteins/genetics*
- Rabbits
- Sequence Alignment
- Zebrafish/genetics*
- Zebrafish Proteins/analysis
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- PubMed
- 20979248 Full text @ Zool. Res.
Citation
Zhao, C.L., Yang, Q.W., Hu, J.R., Ye, D., Gong, W.M., Lv, H.Y., Xu, Z.Y., Zhang, X.P., and Song, P. (2010) Identification of zRAP55,a gene preponderantly expressed in Stages I and II oocytes of zebrafish. Dong wu xue yan jiu = Zoological research. 31(5):469-475.
Abstract
In an in silico search for gonand specific expressed genes, we have identified zRAP55 which is enriched in the ovary of zebrafish . zRAP55 encodes a protein of 382 amino acids with a highly conserved Lsm domain. zRAP55 protein shares more than 56% identities with that of other vertebrate species. RT-PCR results show that it is predominantly expressed in the ovary. In situ hybridization and immunohistochemistry studies reveal that zRAP55 is ubiquitously dispersed throughout the cytoplasm of stages I and II oocytes, whereas no expression is observed in stages III and IV oocytes. As an RNA associated protein, zRAP55 might function in the control of protein translation at the early stages of oogenesis in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping