PUBLICATION

Nab proteins mediate a negative feedback loop controlling Krox-20 activity in the developing hindbrain

Authors
Mechta-Grigoriou, F., Garel, S., and Charnay, P.
ID
ZDB-PUB-101027-49
Date
2000
Source
Development (Cambridge, England)   127(1): 119-128 (Journal)
Registered Authors
Charnay, Patrick
Keywords
none
MeSH Terms
  • 3T3 Cells
  • Animals
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/physiology
  • Early Growth Response Protein 2
  • Feedback
  • Gene Expression Regulation, Developmental*
  • Mice
  • Neoplasm Proteins*
  • Repressor Proteins/genetics*
  • Rhombencephalon/embryology*
  • Rhombencephalon/pathology
  • Transcription Factors/genetics*
  • Transcription Factors/physiology
  • Zebrafish
PubMed
10654606 Full text @ Development
Abstract
The developing vertebrate hindbrain is transiently subdivided along the anterior-posterior axis into metameric units, called rhombomeres (r). These segments constitute units of lineage restriction and display specific gene expression patterns. The transcription factor gene Krox-20 is restricted to r3 and r5, and is required for the development of these rhombomeres. We present evidence that Krox-20 transcriptional activity is under the control of a negative feedback mechanism in the hindbrain. This regulatory loop involves two closely related proteins, Nabl and Nab2, previously identified as antagonists of Krox-20 transcriptional activity in cultured cells. Here we show that in the mouse hindbrain, Nab1 and Nab2 recapitulate the Krox-20 expression pattern and that their expression is dependent on Krox-20 function. Furthermore, misexpression of Nab1 or Nab2 in zebrafish embryos leads to alterations in the expression patterns of several hindbrain markers, consistent with an inhibition of Krox-20 activity. Taken together, these data indicate that Krox-20 positively regulates the expression of its own antagonists and raise the possibility that this negative feedback regulatory loop may play a role in the control of hindbrain development.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping